Overview

  • Product nameAnti-CARD15 antibody
    See all CARD15 primary antibodies
  • Description
    Goat polyclonal to CARD15
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chimpanzee
  • Immunogen

    Synthetic peptide:

    KFRFTDRERHCSPTD

    , corresponding to internal sequence amino acids 385-399 of Human CARD15

  • Positive control
    • Human peripheral blood mononucleocytes.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 115 kDa). An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide.

    Peptide ELISA: antibody detection limit dilution 1:64,000.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInduces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS).
    • Tissue specificityMonocytes-specific.
    • Involvement in diseaseDefects in NOD2 are the cause of Blau syndrome (BS) [MIM:186580]. BS is a rare autosomal dominant disorder characterized by early-onset granulomatous arthritis, uveitis and skin rash.
      Defects in NOD2 are a cause of susceptibility to inflammatory bowel disease type 1 (IBD1) [MIM:266600]. IBD1 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
      Defects in NOD2 are the cause of sarcoidosis early-onset (EOS) [MIM:609464]. EOS is a form of sarcoidosis manifesting in children younger than 4 years of age. Sarcoidosis is an idiopathic, systemic, inflammatory disease characterized by the formation of immune granulomas in involved organs. Granulomas predominantly invade the lungs and the lymphatic system, but also skin, liver, spleen, eyes and other organs may be involved. Early-onset sarcoidosis is quite rare and has a distinct triad of skin, joint and eye disorders, without apparent pulmonary involvement. Compared with an asymptomatic and sometimes naturally disappearing course of the disease in older children, early-onset sarcoidosis is progressive and in many cases causes severe complications, such as blindness, joint destruction and visceral involvement.
    • Sequence similaritiesContains 2 CARD domains.
      Contains 9 LRR (leucine-rich) repeats.
      Contains 1 NACHT domain.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACUG antibody
      • Arthrocutaneouveal granulomatosis antibody
      • BLAU antibody
      • CARD15 antibody
      • Caspase recruitment domain family, member 15 antibody
      • Caspase recruitment domain protein 15 antibody
      • Caspase recruitment domain-containing protein 15 antibody
      • CD antibody
      • CLR16.3 antibody
      • IBD1 antibody
      • Inflammatory bowel disease protein 1 antibody
      • LRR containing protein antibody
      • NLR family, CARD domain containing 2 antibody
      • NLRC2 antibody
      • NOD like receptor C2 antibody
      • NOD2 antibody
      • NOD2 protein antibody
      • NOD2_HUMAN antibody
      • NOD2B antibody
      • nucleotide binding oligomerization domain 2 antibody
      • Nucleotide binding oligomerization domain containing 2 antibody
      • Nucleotide binding oligomerization domain, leucine rich repeat and CARD domain containing 2 antibody
      • Nucleotide-binding oligomerization domain-containing protein 2 antibody
      • PSORAS1 antibody
      see all

    Anti-CARD15 antibody images

    • Anti-CARD15 antibody (ab53518) at 0.1 µg/ml + Human Peripheral Blood Mononucleocyte lysate (35µg protein in RIPA buffer).

      Predicted band size : 115 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-CARD15 antibody (ab53518)

    ab53518 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab53518.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"