• Product nameAnti-CARD4 antibody
    See all CARD4 primary antibodies
  • Description
    Rabbit polyclonal to CARD4
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 698-928 of Human CARD4 (NP_006083).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2 and Raji cell lysates for WB; HeLa cells for IF.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab97278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 108 kDa.
ICC/IF 1/100 - 1/200.


  • FunctionEnhances caspase-9-mediated apoptosis. Induces NF-kappa-B activity via RIPK2 and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Forms an intracellular sensing system along with ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIPK2 dependent NF-kappa-B signaling pathway activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides but also in the activation of NF-kappa-B by Shigella effector proteins IpgB2 and OspB. Recruits NLRP10 to the cell membrane following bacterial infection.
  • Tissue specificityHighly expressed in adult heart, skeletal muscle, pancreas, spleen and ovary. Also detected in placenta, lung, liver, kidney, thymus, testis, small intestine and colon.
  • Sequence similaritiesContains 1 CARD domain.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • Post-translational
    Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF34 promotes proteasomal degradation and thereby negatively regulates NOD1 for instance in NF-kappa-B activition.
  • Cellular localizationCytoplasm. Cell membrane. Apical cell membrane. Basolateral cell membrane. Detected in the cytoplasm and at the cell membrane. Following bacterial infection, localizes to bacterial entry sites in the cell membrane. Recruited to the basolateral and apical membranes in polarized epithelial cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • CARD 4 antibody
    • CARD4 antibody
    • Caspase recruitment domain 4 antibody
    • Caspase recruitment domain containing protein 4 antibody
    • Caspase recruitment domain family member 4 antibody
    • Caspase recruitment domain protein 4 antibody
    • Caspase recruitment domain-containing protein 4 antibody
    • CLR 7.1 antibody
    • CLR7.1 antibody
    • NLR family CARD domain containing 1 antibody
    • NLRC 1 antibody
    • NLRC1 antibody
    • NOD 1 antibody
    • Nod1 antibody
    • NOD1 protein antibody
    • NOD1_HUMAN antibody
    • Nucleotide binding oligomerization domain containing 1 antibody
    • Nucleotide binding oligomerization domain leucine rich repeat and CARD domain containing 1 antibody
    • Nucleotide-binding oligomerization domain-containing protein 1 antibody
    • Protein Nod1 antibody
    see all

Anti-CARD4 antibody images

  • Anti-CARD4 antibody (ab97278) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size : 108 kDa
  • ab97278 at 1/200 dilution staining CARD4 in paraformaldehyde-fixed HeLa cells by immunofluorescence. The lower image shows ab97278 merged with a DNA probe.

References for Anti-CARD4 antibody (ab97278)

ab97278 has not yet been referenced specifically in any publications.

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