Anti-CARD4 antibody - C-terminal (ab170547)

Overview

  • Product name
    Anti-CARD4 antibody - C-terminal
    See all CARD4 primary antibodies
  • Description
    Rabbit polyclonal to CARD4 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human CARD4 aa 930-953 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9Y239

  • Positive control
    • Mouse bladder tissue lysate; HL60 and CEM cell lysates; Human pancreas tissue; Jurkat and HepG2 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 108 kDa.
ICC/IF 1/10 - 1/50.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Enhances caspase-9-mediated apoptosis. Induces NF-kappa-B activity via RIPK2 and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Forms an intracellular sensing system along with ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIPK2 dependent NF-kappa-B signaling pathway activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides but also in the activation of NF-kappa-B by Shigella effector proteins IpgB2 and OspB. Recruits NLRP10 to the cell membrane following bacterial infection.
  • Tissue specificity
    Highly expressed in adult heart, skeletal muscle, pancreas, spleen and ovary. Also detected in placenta, lung, liver, kidney, thymus, testis, small intestine and colon.
  • Sequence similarities
    Contains 1 CARD domain.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • Post-translational
    modifications
    Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF34 promotes proteasomal degradation and thereby negatively regulates NOD1 for instance in NF-kappa-B activition.
  • Cellular localization
    Cytoplasm. Cell membrane. Apical cell membrane. Basolateral cell membrane. Detected in the cytoplasm and at the cell membrane. Following bacterial infection, localizes to bacterial entry sites in the cell membrane. Recruited to the basolateral and apical membranes in polarized epithelial cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • CARD 4 antibody
    • CARD4 antibody
    • Caspase recruitment domain 4 antibody
    • Caspase recruitment domain containing protein 4 antibody
    • Caspase recruitment domain family member 4 antibody
    • Caspase recruitment domain protein 4 antibody
    • Caspase recruitment domain-containing protein 4 antibody
    • CLR 7.1 antibody
    • CLR7.1 antibody
    • NLR family CARD domain containing 1 antibody
    • NLRC 1 antibody
    • NLRC1 antibody
    • NOD 1 antibody
    • Nod1 antibody
    • NOD1 protein antibody
    • NOD1_HUMAN antibody
    • Nucleotide binding oligomerization domain containing 1 antibody
    • Nucleotide binding oligomerization domain leucine rich repeat and CARD domain containing 1 antibody
    • Nucleotide-binding oligomerization domain-containing protein 1 antibody
    • Protein Nod1 antibody
    see all

Images

  • Flow cytometric analysis of Jurkat cells labeling CARD4 with ab170547 at 1/10 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

  • Immunofluorescent analysis of  HepG2 cells labeling CARD4 with ab170547 at 1/10 dilution (green) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG. DAPI was used to stain the cell nuclear (blue).

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human pancreas tissue labeling CARD4 with ab170547 at 1/10 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.

  • All lanes : Anti-CARD4 antibody - C-terminal (ab170547) at 1/100 dilution

    Lane 1 : HL60 cell lysate
    Lane 2 : CEM cell lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    All lanes : Anti-rabbit HRP conjugated antibody at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 108 kDa

  • Anti-CARD4 antibody - C-terminal (ab170547) at 1/100 dilution + Mouse bladder tissue lysate at 35 µg

    Secondary
    Anti-rabbit HRP conjugated antibody at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 108 kDa

References

ab170547 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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