The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: peptide ELISA (antibody detection limit dilution >1 / 32000).
IHC-P: Use at a concentration of 5 µg/ml.
WB: Approx. 45-50 kDa and 25-30 kDa bands observed in A549, Human Heart and Human Muscle lysates at 1ug of primary. Please note that the band we observe is different from the predicted size of approx. 119 kDa according to NP_115976.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ab4381 at 5ug/ml staining CARD6 in human pancreas tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). A AP-staining procedure was used for detection.
References for Anti-CARD6 antibody (ab4381)
has not yet been referenced specifically in any publications.
Publishing research using ab4381? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"