Overview

  • Product nameAnti-CARD8 antibody [2108C2a]
    See all CARD8 primary antibodies
  • Description
    Mouse monoclonal [2108C2a] to CARD8
  • Tested applicationsSuitable for: WB, IP, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human CARD8 fragment

  • Epitopeab50977 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
  • Positive control
    • HeLa whole cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, 8.0mM Sodium phosphate, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number2108C2a
  • IsotypeIgG1
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab50977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
Dot blot
  • Application notesDot: Use at an assay dependent dilution.
    IP: Use 5µg/200ul HeLa RIPA lysate.
    WB: Use at an assay dependent dilution. Detects a band of approximately 46 kDa (predicted molecular weight: 49 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInhibits NF-kappa-B activation. May participate in a regulatory mechanism that coordinates cellular responses controlled by NF-kappa-B transcription factor. May be a component of the inflammasome, a protein complex which also includes PYCARD, NALP2 and CASP1 and whose function would be the activation of proinflammatory caspases.
    • Tissue specificityHigh expression in lung, ovary, testis and placenta. Lower expression in heart, kidney and liver. Also expressed in spleen, lymph node and bone marrow.
    • Sequence similaritiesContains 1 CARD domain.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Apoptotic protein NDPP 1 antibody
      • Apoptotic protein NDPP1 antibody
      • CARD 8 antibody
      • CARD inhibitor of NF kappa B activating ligand antibody
      • CARD inhibitor of NF kappaB activating ligand antibody
      • CARD inhibitor of NF kappaB activating ligands antibody
      • CARD-inhibitor of NF-kappa-B-activating ligand antibody
      • CARD8 antibody
      • CARD8_HUMAN antibody
      • CARDINAL antibody
      • Caspase recruitment domain containing protein 8 antibody
      • Caspase recruitment domain family member 8 antibody
      • Caspase recruitment domain protein 8 antibody
      • Caspase recruitment domain-containing protein 8 antibody
      • DACAR antibody
      • Dakar antibody
      • DKFZp779L0366 antibody
      • FLJ18119 antibody
      • FLJ18121 antibody
      • KIAA0955 antibody
      • MGC57162 antibody
      • NDPP 1 antibody
      • NDPP antibody
      • NDPP1 antibody
      • TUCAN antibody
      • Tumor up regulated CARD containing antagonist of CASP9 antibody
      • Tumor up regulated CARD containing antagonist of caspase 9 antibody
      • Tumor up-regulated CARD-containing antagonist of CASP9 antibody
      • tumor up-regulated CARD-containing antagonist of caspase nine antibody
      • Tumor upregulated CARD containing antagonist of CASP9 antibody
      see all

    Anti-CARD8 antibody [2108C2a] images

    • Anti-CARD8 antibody [2108C2a] (ab50977) at 1/200 dilution + HeLa whole cell lysate at 25 µg

      Secondary
      anti-mouse IgG at 1/2500 dilution
      Developed using the ECL technique

      Predicted band size : 49 kDa
      Observed band size : 46 kDa (why is the actual band size different from the predicted?)
    • Immunoprecipitaion analysis uisng ab50977 at 5ug per 200ul HeLa cell RIPA lysate. Immunoblot: ab50977 at 1/200 dilution using 1/1000 anti-mouse IgG as a secondary antibody.

    References for Anti-CARD8 antibody [2108C2a] (ab50977)

    ab50977 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab50977.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"