Overview

  • Product nameAnti-CARD9 antibody [EPR6488]
    See all CARD9 primary antibodies
  • Description
    Rabbit monoclonal [EPR6488] to CARD9
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • THP1, Raji, Jurkat, and PC3 cell lysates; Human brain and liver tissues
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD) KD = 1.79 x 10 -10 M Learn more about KD
  • Storage bufferpH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR6488
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • FunctionActivates NF-kappa-B via BCL10.
    • Tissue specificityHighly expressed in spleen. Also detected in liver, placenta, lung, peripheral blood leukocytes and in brain.
    • Involvement in diseaseDefects in CARD9 are the cause of familial candidiasis type 2 (CANDF2) [MIM:212050]. Chronic mucocutaneous candidiasis is characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CANDF2 is an autosomal recessive chronic mucocutaneous candidiasis.
    • Sequence similaritiesContains 1 CARD domain.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • CANDF2 antibody
      • CARD9 antibody
      • CARD9_HUMAN antibody
      • Caspase recruitment domain family member 9 antibody
      • Caspase recruitment domain-containing protein 9 antibody
      • hCARD9 antibody
      see all

    Anti-CARD9 antibody [EPR6488] images

    • All lanes : Anti-CARD9 antibody [EPR6488] (ab124922) at 1/1000 dilution

      Lane 1 : THP1 cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : PC3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 62 kDa
      Observed band size : 62 kDa
    • ab124922, at 1/250 dilution, staining CARD9 in paraffin-embedded Human brain tissue by Immunohistochemistry.
    • ab124922, at 1/250 dilution, staining CARD9 in paraffin-embedded Human liver tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-CARD9 antibody [EPR6488] (ab124922)

    ab124922 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab124922.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"