cardiac Myosin type II antibody [BGN/04/4482] (ab58913)

Product Name 

cardiac Myosin type II antibody [BGN/04/4482]

Product type 

Primary antibodies

Description 

Mouse monoclonal [BGN/04/4482] to cardiac Myosin type II

Immunogen 

Myosin heavy chain from rabbit skeletal muscle.

Reacts with 
(species key)

Rb

Tested applications 
(see key)

ELISA, IHC-Fr, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ELISA: 1/5000 - 1/20000.
IHC-Fr: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 223 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasm, myofibril. Note: thick filaments of the myofibrils.

Research areas 

Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Myosins
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Myosin

Relevance 

Most myosin molecules are composed of both a head and a tail domain. The head domain binds the filamentous actin, and uses ATP hydrolysis to generate force and to "walk" along the filament towards the (+) end (with the exception of one family member, myosin VI, which moves towards the (-) end). The tail domain generally mediates interaction with cargo molecules and/or other myosin subunits. Myosin II, responsible for skeletal muscle contraction, is perhaps the best-studied example of these properties. Myosin II contains two heavy chains, each about 2000 amino acids in length, which constitute the head and tail domains. Each of these heavy chains contains the N-terminal head domain, while the C-terminal tails take on a coiled-coil morphology, holding the two heavy chains together. Thus, myosin II has two heads. It also contains 4 light chains (2 per head), which bind the heavy chains in the "neck" region between the head and tail. In muscle cells, it is myosin II that is responsible for producing the contractile force.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

BGN/04/4482

Isotype 

IgG1

Purity 

Protein A purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

2.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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