Anti-Cardiac Troponin I antibody [22B11] (HRP) (ab8290)

Overview

  • Product nameAnti-Cardiac Troponin I antibody [22B11] (HRP)
    See all Cardiac Troponin I primary antibodies
  • Description
    Mouse monoclonal [22B11] to Cardiac Troponin I (HRP)
  • ConjugationHRP
  • SpecificityThis antibody detects the unphosphorylated form of cardiac troponin I. No cross-reaction with mono or biphosphorylated troponin I.
  • Tested applicationsELISA, WBmore details
  • Species reactivity
    Reacts with: Human, Pig

    Does not react with

    Mouse, Rat, Rabbit, Goat, Cow, Cat, Dog, Fish
  • Immunogen

    Unphosphorylated form of cardiac troponin I (amino acid residues 20-24)

  • Epitope20-24 aa

Properties

Applications

Our Abpromise guarantee covers the use of ab8290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 25 kDa.

Target

  • FunctionTroponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Involvement in diseaseDefects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similaritiesBelongs to the troponin I family.
  • Information by UniProt
  • Database links
  • Alternative names
    • cardiac muscle antibody
    • Cardiac troponin I antibody
    • Cardiomyopathy, familial hypertrophic, 7, included antibody
    • CMD1FF antibody
    • CMD2A antibody
    • CMH7 antibody
    • cTnI antibody
    • Familial hypertrophic cardiomyopathy 7 antibody
    • MGC116817 antibody
    • RCM1 antibody
    • Tn1 antibody
    • Tni antibody
    • TNN I3 antibody
    • TNNC 1 antibody
    • TNNC1 antibody
    • TNNI3 antibody
    • TNNI3 antibody
    • TNNI3_HUMAN antibody
    • Troponin I antibody
    • Troponin I cardiac antibody
    • Troponin I cardiac muscle antibody
    • Troponin I cardiac muscle isoform antibody
    • Troponin I type 3 cardiac antibody
    • troponin I, cardiac 3 antibody
    • TroponinI antibody
    • Ttroponin I type 3 (cardiac) antibody
    see all

Anti-Cardiac Troponin I antibody [22B11] (HRP) images

  • Two sandwich ELISA tested antibody combinations, clone 19C7 and ab10225 (clone 10B11), and clone 19C7 and ab8290 (clone 22B11) showing differing recognization of dephospho-(dark blue columns) and biphospho-(light blue columns) forms of cTnI.
  • Analysis from ELISA results. cTnI measurements in total (blue circles) and dephosphorylated (grey squares). Taken from cTnI in serial blood samples from an AMI patient. Antibodies used were clone 8E10, ab8290 (clone 22B11), clone 19C7.

References for Anti-Cardiac Troponin I antibody [22B11] (HRP) (ab8290)

This product has been referenced in:
  • Gallon CE Current techniques for the study of troponin I phosphorylation in human heart. J Muscle Res Cell Motil 29:169-72 (2008). WB ; Human . Read more (PubMed: 19172403) »

See 1 Publication for this product

Product Wall

Thank you for your email. The ab9936 product is a peptide which can be used to block antibodies ab7829, ab8290 and ab8291. This peptide can be used to block these antibodies irrespective of applications e.g. It is suitable to use with antibodies...

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Thank you for your email. Ab8290 does NOT react with rabbit, and ab8291 has only so far been tested for cross-reactivity with human (not yet tested in rabbit). I unfortunately cannot find another cardiac Troponin I antibody in our catalog that is s...

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Amino acid residues 20-24

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"