cardiac Troponin I antibody [2D5] (ab7829)

Product Name 

cardiac Troponin I antibody [2D5]

Product type 

Primary antibodies

Description 

Mouse monoclonal [2D5] to cardiac Troponin I

Immunogen 

Highly purified human cardiac troponin I.

Reacts with 
(species key)

Hu

Specificity 

This antibody is specific for human cardiac troponin subunit I, a 21 kD protein responsible for inhibiting actomyosin Mg 2+ -ATPase activity.

Tested applications 
(see key)

ELISA, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ELISA: 1/500 - 1/1000.
IP: Use at an assay dependent dilution.
WB: 1/50 - 1/100. Predicted molecular weight: 21-23.8 kDa.
IHC-P: 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol:
1) High temp. antigen demasking for FFPE tissue sections.
Deparaffinize and rehydrate tissue sections. Use 10mM citrate buffer pH 6.0, boil 10-20 mins, followed by cooling 10-20 mins
OR
2) Process tissue sections in Pressure cooker for 5 mins in citrate buffer followed by cooling 10-20 mins to RT.Wash tissue sections with PBS.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Developmental Biology >> Lineage specification >> Mesoderm
Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Troponin
Stem Cells >> Lineage Markers >> Mesoderm
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Troponin

Relevance 

Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium.

This antibody reacts with subunit I of the troponin complex in human myocardium, where it is the only troponin I isotype present. It does not cross-react with troponin I of skeletal muscle cells.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

2D5

Isotype 

IgG1

Purity 

Tissue culture supernatant

Storage buffer 

20 mM tris-borate, 150 mM sodium chloride,0.1% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

References for cardiac Troponin I antibody [2D5] (ab7829)

  This product has been used in: (two most recent references)

Xia X et al. In vitro- and in vivo-induced transgene expression in human embryonic stem cells and derivatives. Stem Cells 26:525-33 (2008). IHC; Human. PubMed: 18032700

Canaider S et al. Proteins encoded by human Down syndrome critical region gene 1-like 2 (DSCR1L2) mRNA and by a novel DSCR1L2 mRNA isoform interact with cardiac troponin I (TNNI3). Gene 372:128-36 (2006). PubMed: 16516408
If you publish research using ab7829 please let us know so that we can cite the reference on this datasheet.

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