Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Troponin
Overview
Product name
Anti-Cardiac Troponin I antibody
See all Cardiac Troponin I products (39) ...
Description
Rabbit polyclonal to Cardiac Troponin I
Tested applications
WB, ICC/IF, IHC-P, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Pig
Predicted to work with
Chimpanzee
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human cardiac Troponin I.
(Peptide available as ab47002.)
Positive control
This antibody gave a positive signal in the following tissue lysates: Heart (Human) Tissue Lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Cardiovascular Markers >> Cell Markers >> Cardiomyocytes
Cardiovascular >> Cardiovascular Markers
Developmental Biology >> Lineage specification >> Mesoderm
Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Troponin
Stem Cells >> Lineage Markers >> Mesoderm
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Troponin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab47003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 26 kDa (predicted molecular weight: 24 kDa).
ICC/IF: Use at an assay dependent concentration.
IHC-P: 1/100
Flow Cyt: Use at an assay dependent concentration. (PubMed: 21909276)
Target
Function
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Involvement in disease
Defects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Sequence similarities
Belongs to the troponin I family.
Target information above from: UniProt accessionP19429
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- cardiac muscle antibody
- Cardiac troponin I antibody
- Cardiomyopathy, familial hypertrophic, 7, included antibody
see all
Database links
- Entrez Gene: 7137 Human
- Entrez Gene: 21954 Mouse
- Entrez Gene: 29248 Rat
- Omim: 191044 Human
- SwissProt: P19429 Human
- SwissProt: P48787 Mouse
- SwissProt: P23693 Rat
- Unigene: 709179 Human
see all
Anti-Cardiac Troponin I antibody images:
Western blot - cardiac Troponin I antibody (ab47003)
All lanes : Anti-Cardiac Troponin I antibody (ab47003) at 1 µg/ml
Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)
Lane 2 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 24 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
Western blot - Cardiac Troponin I antibody (ab47003)
All lanes : Anti-Cardiac Troponin I antibody (ab47003) at 1 µg/ml
Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 24 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
Additional bands at : 160 kDa,80 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
Western blot - Cardiac Troponin I antibody (ab47003)
All lanes : Anti-Cardiac Troponin I antibody (ab47003) at 1/20000 dilution
Lane 1 : Pig Heart whole cell lysate
Lane 2 : Pig Heart whole cell lysate
Lane 3 : Pig Heart whole cell lysate
Lane 4 : Pig Heart whole cell lysate
Lysates/proteins at 4 µg per lane.
Secondary
An HRP-conjugated Goat anti-rabbit polyclonal at 1/20000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 24 kDa
Observed band size : 29 kDa (why is the actual band size different from the predicted?)
Blocking Step: 20% Serum for 3 hours at 24°C
This image is courtesy of an Abreview submitted by Dr. Mario Torrado
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cardiac Troponin I antibody (ab47003)
IHC image of Cardiac Troponin I staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47003, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
References for Anti-Cardiac Troponin I antibody (ab47003)
This product has been referenced in:
- Fogle RLet al. Functional proteomic analysis reveals sex-dependent differences in structural and energy-producing myocardial proteins in rat model of alcoholic cardiomyopathy. Physiol Genomics 43:346-56 (2011). WB; Rat.Read more (PubMed: 21245415) »
- Opitz Eet al. Impairment of Immunoproteasome Function by ß5i/LMP7 Subunit Deficiency Results in Severe Enterovirus Myocarditis. PLoS Pathog 7:e1002233 (2011). Flow Cyt; Mouse.Read more (PubMed: 21909276) »
See all 4 publications for this product
Publishing research using ab47003? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"