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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Troponin
Overview
Product name
Cardiac Troponin I protein
See all Cardiac Troponin I products (5) ...
Properties
Purification notes
SDS-PAGE - Major 24 kDa band (> 95%) Source:Human cardiac tissue Purified by Tn-C affinity chromatography
Biological activity
SDS PAGE profile of a typical recombinant Troponin-I preparation (3.8ug) showed a major band at 24kDa and a minor band attributable to Troponin-I dimer. Please note: Troponin-I is very susceptible to proteolytic breakdown within cardiac tissue and it is difficult to isolate undegraded native human cardiac troponin-I. This product is greater than 70% intact and hence proteolytic fragments can be observed.
Form
Liquid
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
8 M Urea, 50 mM Tris.HCl, 15 mM ß-mercaptoethanol, 1 mM EDTA (pH 8.0)
Concentration
Concentration information loading...
Additional notes
Native Human Cardiac Troponin-I
Research areas
Cardiovascular >> Cardiovascular Markers >> Cell Markers >> Cardiomyocytes
Cardiovascular >> Cardiovascular Markers
Developmental Biology >> Lineage specification >> Mesoderm
Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Troponin
Stem Cells >> Lineage Markers >> Mesoderm
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Troponin
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Western blot - cardiac Troponin I protein (ab9936)
(enlarge)
Applications
Protein info
Function
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Involvement in disease
Defects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Sequence similarities
Belongs to the troponin I family.
Target information above from: UniProt accessionP19429
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Cardiac Troponin I protein images:
Western blot - cardiac Troponin I protein (ab9936)
Cardiac Troponin I protein (ab9936) at 2.7 µg (Coomassie blue staining of SDS gel)
Performed under reducing conditions.
Observed band size : 24 kDa (why is the actual band size different from the predicted?)
Additional bands at : 48 kDa (possible dimer).
References for Cardiac Troponin I protein (ab9936)
ab9936 has not yet been referenced specifically in any publications.
Publishing research using ab9936? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"