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Full length native protein (purified) (Human).
This antibody can be used to detect ischemia areas of human heart. It is also useful as marker for brain damage.
Our Abpromise guarantee covers the use of ab16916 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 23029183|
|IP||Use at an assay dependent concentration.|
|IHC-P||1/10. PubMed: 19825952|
|WB||Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 15 kDa.|
IHC image of cardiac FABP staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16916, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.