Anti-Cardiac FABP antibody [67D3] (ab16916)

Overview

  • Product nameAnti-Cardiac FABP antibody [67D3]
    See all Cardiac FABP primary antibodies
  • Description
    Mouse monoclonal [67D3] to Cardiac FABP
  • SpecificityAb16916 binds to human cardiac FABP. It shows no binding to human intestinal or liver FABP and slightly cross reacts with B-FABP.
  • Tested applicationsSuitable for: IHC-Fr, IP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length native protein (purified) (Human).

  • Positive control
    • Heart cells, recombinant human H-FABP This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.
  • General notes


    This antibody can be used to detect ischemia areas of human heart. It is also useful as marker for brain damage.

Properties

Applications

Our Abpromise guarantee covers the use of ab16916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 23029183
IP Use at an assay dependent concentration.
IHC-P 1/10. PubMed: 19825952
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 15 kDa.

Target

  • FunctionFABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.
  • Sequence similaritiesBelongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • DomainForms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 422 protein antibody
    • Cardiac Fatty Acid Binding Protein antibody
    • FABP 11 antibody
    • FABP 3 antibody
    • FABP11 antibody
    • FABP3 antibody
    • FABPH_HUMAN antibody
    • Fatty acid binding protein 11 antibody
    • Fatty acid binding protein 3 antibody
    • Fatty acid binding protein 3 muscle and heart antibody
    • Fatty acid binding protein 3 muscle and heart mammary derived growth inhibitor antibody
    • Fatty acid binding protein 3 muscle antibody
    • Fatty acid binding protein 3, muscle and heart (mammary derived growth inhibitor) antibody
    • Fatty acid binding protein 3, muscle antibody
    • Fatty acid binding protein heart antibody
    • Fatty acid binding protein, heart antibody
    • Fatty acid binding protein, muscle and heart antibody
    • Fatty acid binding protein, skeletal muscle antibody
    • Fatty acid-binding protein 3 antibody
    • Fatty acid-binding protein antibody
    • H FABP antibody
    • H-FABP antibody
    • heart antibody
    • Heart type fatty acid binding protein antibody
    • Heart-type fatty acid-binding protein antibody
    • M FABP antibody
    • M-FABP antibody
    • Mammary derived growth inhibitor antibody
    • Mammary-derived growth inhibitor antibody
    • MDGI antibody
    • Muscle fatty acid binding protein antibody
    • Muscle fatty acid-binding protein antibody
    • Mylein protein P2 homolog antibody
    • O FABP antibody
    • OTTHUMP00000003898 antibody
    • P2 adopocyte protein antibody
    see all

Anti-Cardiac FABP antibody [67D3] images

  • IHC image of cardiac FABP staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16916, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Cardiac FABP antibody [67D3] (ab16916)

This product has been referenced in:
  • Jones HB  et al. Myocardial Steatosis and Necrosis in Atria and Ventricles of Rats Given Pyruvate Dehydrogenase Kinase Inhibitors. Toxicol Pathol N/A:N/A (2014). Read more (PubMed: 24742628) »
  • Chen HM  et al. Heart-type Fatty Acid binding protein is associated with proteinuria in obesity. PLoS One 7:e45691 (2012). IHC-P, IHC-Fr ; Mouse . Read more (PubMed: 23029183) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Heart)
Specification Heart
Fixative Formaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum free as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 0µg/mL · Temperature: 24°C
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Abcam user community

Verified customer

Submitted Feb 26 2009

Thank you for your recent telephone enquiry. I have received confirmation that this antibody is cross reactive with rat and mouse H-FABP (Isoform FABP3). I hope this information is helpful. Should you have any further questions, please do not h...

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Thank you for your enquiry regarding ab16916. The monoclonal antibody binds to human heart FABP. It shows no binding to human intestinal or liver FABP. Western blot analysis showed that the antibody slightly cross reacts with B-FABP. For immunoh...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"