Overview

  • Product nameAnti-Cardiac Troponin C antibody [1A2]
    See all Cardiac Troponin C primary antibodies
  • Description
    Mouse monoclonal [1A2] to Cardiac Troponin C
  • Tested applicationsSuitable for: IHC-P, Other, Sandwich ELISA, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab8285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
Other Use at an assay dependent concentration.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [7B9] to Cardiac Troponin C (HRP) (ab8286). Can be used as Capture antibody.
AP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Target

  • FunctionTroponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.
  • Involvement in diseaseDefects in TNNC1 are the cause of cardiomyopathy dilated type 1Z (CMD1Z) [MIM:611879]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNC1 are the cause of familial hypertrophic cardiomyopathy type 13 (CMH13) [MIM:613243]. A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
  • Sequence similaritiesBelongs to the troponin C family.
    Contains 4 EF-hand domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • tnnc1a antibody
    • Cardiac troponin C antibody
    • slow skeletal and cardiac muscles antibody
    • TN-C antibody
    • TNC antibody
    • Tnnc1 antibody
    • TNNC1_HUMAN antibody
    • TNNI3 antibody
    • Troponin C antibody
    • Troponin C slow antibody
    • Troponin C1 slow antibody
    see all

Anti-Cardiac Troponin C antibody [1A2] images

  • Ab8285 staining Human normal skeletal muscle. Staining is localised to the cytoplasm.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-Cardiac Troponin C antibody [1A2] (ab8285)

This product has been referenced in:
  • Bedada FB  et al. Acquisition of a quantitative, stoichiometrically conserved ratiometric marker of maturation status in stem cell-derived cardiac myocytes. Stem Cell Reports 3:594-605 (2014). Read more (PubMed: 25358788) »
  • Willis MS  et al. Cardiac muscle ring finger-1 increases susceptibility to heart failure in vivo. Circ Res 105:80-8 (2009). WB ; Mouse . Read more (PubMed: 19498199) »

See all 3 Publications for this product

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Cross reactivity with other species has not yet been determined

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"