Overview

  • Product nameAnti-Cardiac Troponin C antibody
    See all Cardiac Troponin C primary antibodies
  • Description
    Goat polyclonal to Cardiac Troponin C
  • SpecificityThis antibody recognizes cardiac Troponin C. It shows no reactivity with Troponin T.
  • Tested applicationsSuitable for: ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Chicken, Cow, Pig, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide:

    FVLG AEDGCISTKE

    , corresponding to amino acids 27-40 of Human cardiac Troponin C.

Properties

Applications

Our Abpromise guarantee covers the use of ab30807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionTroponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.
  • Involvement in diseaseDefects in TNNC1 are the cause of cardiomyopathy dilated type 1Z (CMD1Z) [MIM:611879]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNC1 are the cause of familial hypertrophic cardiomyopathy type 13 (CMH13) [MIM:613243]. A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
  • Sequence similaritiesBelongs to the troponin C family.
    Contains 4 EF-hand domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • tnnc1a antibody
    • Cardiac troponin C antibody
    • slow skeletal and cardiac muscles antibody
    • TN-C antibody
    • TNC antibody
    • Tnnc1 antibody
    • TNNC1_HUMAN antibody
    • TNNI3 antibody
    • Troponin C antibody
    • Troponin C slow antibody
    • Troponin C1 slow antibody
    see all

Anti-Cardiac Troponin C antibody images

  • ab30807 staining Cardiac Troponin C in mouse heart tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone, permeabilized with 0.3% Triton in PBS and blocked with 1% BSA + 5% donkey serum for 45 minutes at room temperature. Samples were incubated with primary antibody (1/500 in PBS + 0.3% Triton + 0.1% BSA + 5% donkey serum) for 16 hours at 4°C. An Alexa Fluor® 594-conjugated donkey anti-goat IgG polyclonal (1/300) was used as the secondary antibody.

    Right - goat isotype control.

    See Abreview

References for Anti-Cardiac Troponin C antibody (ab30807)

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step 0.1% BSA and 5% Donkey serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Mouse Tissue sections (mouse heart)
Specification mouse heart
Permeabilization Yes - 0.3% Triton PBS
Fixative Acetone
Username

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Verified customer

Submitted Jan 30 2015

Thanks for your inquiry. Concerning ab30807, Troponin I, Cardiac, aa27-40 (cTnI) Pab Goat x Human: Crossreactivity of this antibody with human skeletal Troponin I is ~0.02% by ELISA. Please let me know if you require further a...

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