Anti-Cardiac Troponin I antibody [10B11] (ab10225)

Overview

  • Product nameAnti-Cardiac Troponin I antibody [10B11]
    See all Cardiac Troponin I primary antibodies
  • Description
    Mouse monoclonal [10B11] to Cardiac Troponin I
  • SpecificityReacts with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components (in the presence of 5 mM EDTA). Not affected by heparin, phosphorylation, oxidation and troponin complex formation. Recognizes dephospho- and phospho- forms of human cardiac Troponin I. Does not cross-react with skeletal muscle troponin I.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Rabbit, Cat, Dog, Human, Pig
    Does not react with: Mouse, Goat, Cow, Fish
  • Immunogen

    Full length protein (Human).

  • Epitope16-20 aa.
  • General notesConcentration varies from lot to lot and can be provided on request.


    cardiac Troponin I is phosphorylated in vivo and it can also be phosphorylated in vitro by cAMP-dependent protein kinase A. Sites of phosphorylation are the serines in the 22nd and 23rd position (Jaquet K et al). ab10225 recognizes the epitopes containing these two amino acid residues.

Properties

Applications

Our Abpromise guarantee covers the use of ab10225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
AP Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 24 kDa.

Target

  • FunctionTroponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Involvement in diseaseDefects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similaritiesBelongs to the troponin I family.
  • Information by UniProt
  • Database links
  • Alternative names
    • cardiac muscle antibody
    • Cardiac troponin I antibody
    • cardiomyopathy, dilated 2A (autosomal recessive) antibody
    • Cardiomyopathy, familial hypertrophic, 7, included antibody
    • CMD1FF antibody
    • CMD2A antibody
    • CMH7 antibody
    • cTnI antibody
    • Familial hypertrophic cardiomyopathy 7 antibody
    • MGC116817 antibody
    • RCM1 antibody
    • Tn1 antibody
    • Tni antibody
    • TNN I3 antibody
    • TNNC 1 antibody
    • TNNC1 antibody
    • TNNI3 antibody
    • TNNI3_HUMAN antibody
    • Troponin I antibody
    • Troponin I cardiac antibody
    • Troponin I cardiac muscle antibody
    • Troponin I cardiac muscle isoform antibody
    • Troponin I type 3 cardiac antibody
    • troponin I, cardiac 3 antibody
    • TroponinI antibody
    • Ttroponin I type 3 (cardiac) antibody
    see all

Anti-Cardiac Troponin I antibody [10B11] images

  • Two sandwich ELISA tested antibody combinations, clone 19C7 and ab10225 (clone 10B11), and clone 19C7 and ab8290 (clone 22B11) showing differing recognization of dephospho-(dark blue columns) and biphospho-(light blue columns) forms of cTnI.

References for Anti-Cardiac Troponin I antibody [10B11] (ab10225)

ab10225 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. To use ab10225 in Western blotting, I would recommend starting at a dilution of 1:1000 and optimizing from there. If you have any more questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"