Overview

  • Product nameAnti-Cardiac Troponin I antibody [19C7]
    See all Cardiac Troponin I primary antibodies
  • Description
    Mouse monoclonal [19C7] to Cardiac Troponin I
  • SpecificityNo cross-reaction with skeletal muscle Troponin I
  • Tested applicationsSuitable for: WB, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chicken, Xenopus laevis
  • Immunogen

    Synthetic peptide:

    ISASRKLQL

    , corresponding to amino acids 41-49 of Human Cardiac Troponin I

  • EpitopeRecognises an epitope included within aa 41-49

Properties

Applications

Our Abpromise guarantee covers the use of ab92408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [4C2] to Cardiac Troponin I (ab10231) and Mouse monoclonal [16A11] to Cardiac Troponin I (HRP) (ab24460).

Recommended pairs for quantitative sandwich immunoassay (capture-detection):
ab92408 – ab24460, ab10231 – ab92408

Target

  • FunctionTroponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Involvement in diseaseDefects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similaritiesBelongs to the troponin I family.
  • Information by UniProt
  • Database links
    • Alternative names
      • cardiac muscle antibody
      • Cardiac troponin I antibody
      • cardiomyopathy, dilated 2A (autosomal recessive) antibody
      • Cardiomyopathy, familial hypertrophic, 7, included antibody
      • CMD1FF antibody
      • CMD2A antibody
      • CMH7 antibody
      • cTnI antibody
      • Familial hypertrophic cardiomyopathy 7 antibody
      • MGC116817 antibody
      • RCM1 antibody
      • Tn1 antibody
      • Tni antibody
      • TNN I3 antibody
      • TNNC 1 antibody
      • TNNC1 antibody
      • TNNI3 antibody
      • TNNI3_HUMAN antibody
      • Troponin I antibody
      • Troponin I cardiac antibody
      • Troponin I cardiac muscle antibody
      • Troponin I cardiac muscle isoform antibody
      • Troponin I type 3 cardiac antibody
      • troponin I, cardiac 3 antibody
      • TroponinI antibody
      • Ttroponin I type 3 (cardiac) antibody
      see all

    References for Anti-Cardiac Troponin I antibody [19C7] (ab92408)

    This product has been referenced in:
    • Clause KC  et al. Developing cardiac and skeletal muscle share fast-skeletal myosin heavy chain and cardiac troponin-I expression. PLoS One 7:e40725 (2012). WB, IHC (PFA fixed) . Read more (PubMed: 22808244) »

    See 1 Publication for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (iPSC-derived cardiomyocytes)
    Permeabilization Yes - 10 minutes in 0.2% Triton X-100
    Specification iPSC-derived cardiomyocytes
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative Paraformaldehyde
    Username

    Mr. Mick Wauchope

    Verified customer

    Submitted Mar 23 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"