Anti-Cardiac Troponin I antibody [4E5] (ab125699)

Overview

  • Product nameAnti-Cardiac Troponin I antibody [4E5]
    See all Cardiac Troponin I primary antibodies
  • Description
    Mouse monoclonal [4E5] to Cardiac Troponin I
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Dog, Human, Monkey
  • Immunogen

    Recombinant full length Human Cardiac Troponin I produced in E.coli (NP_000354).

  • Positive control
    • HEK293T cells transfected with pCMV6-ENTRY Cardiac Troponin I cDNA; HepG2, HeLa, HT29, A549, COS7, Jurkat, MDCK, and MCF7 cell extracts; COS7 cells transiently transfected by pCMV6-ENTRY Cardiac Troponin I; HeLa and A549 cells.
  • General notesDilute in PBS (pH7.3) before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab125699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2500 - 1/5000. Predicted molecular weight: 24 kDa.
ICC/IF 1/50 - 1/100.

Target

  • FunctionTroponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Involvement in diseaseDefects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similaritiesBelongs to the troponin I family.
  • Information by UniProt
  • Database links
  • Alternative names
    • cardiac muscle antibody
    • Cardiac troponin I antibody
    • cardiomyopathy, dilated 2A (autosomal recessive) antibody
    • Cardiomyopathy, familial hypertrophic, 7, included antibody
    • CMD1FF antibody
    • CMD2A antibody
    • CMH7 antibody
    • cTnI antibody
    • Familial hypertrophic cardiomyopathy 7 antibody
    • MGC116817 antibody
    • RCM1 antibody
    • Tn1 antibody
    • Tni antibody
    • TNN I3 antibody
    • TNNC 1 antibody
    • TNNC1 antibody
    • TNNI3 antibody
    • TNNI3_HUMAN antibody
    • Troponin I antibody
    • Troponin I cardiac antibody
    • Troponin I cardiac muscle antibody
    • Troponin I cardiac muscle isoform antibody
    • Troponin I type 3 cardiac antibody
    • troponin I, cardiac 3 antibody
    • TroponinI antibody
    • Ttroponin I type 3 (cardiac) antibody
    see all

Anti-Cardiac Troponin I antibody [4E5] images

  • All lanes : Anti-Cardiac Troponin I antibody [4E5] (ab125699) at 1/2500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Cardiac Troponin I cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 24 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC214740)
  • All lanes : Anti-Cardiac Troponin I antibody [4E5] (ab125699) at 1/2500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : HT29 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 24 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC214740)
  • ab125699 at 1/50 dilution staining Cardiac Troponin I in COS7 cells transiently transfected with pCMV6-ENTRY Cardiac Troponin I by Immunofluorescence.
  • ab125699 at 1/50 dilution staining Cardiac Troponin I in HeLa cells by Immunofluorescence.
  • ab125699 at 1/50 dilution staining Cardiac Troponin I in Jurkat cells by Immunofluorescence.

References for Anti-Cardiac Troponin I antibody [4E5] (ab125699)

ab125699 has not yet been referenced specifically in any publications.

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Thank you foryour telelephone call earlier today.

As discussed on the telephone, I am sorry to confirm that as we are aware, ab125699Cardiac Troponin I antibody [4E5]] has never been tested with samples from mouse. All tested species cross-r...

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