Cardiac Troponin I full length protein (ab9936)

Overview

Description

  • NatureRecombinant
  • SourceNative

Specifications

Our Abpromise guarantee covers the use of ab9936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySDS PAGE profile of a typical recombinant Troponin-I preparation (3.8ug) showed a major band at 24kDa and a minor band attributable to Troponin-I dimer. Please note: Troponin-I is very susceptible to proteolytic breakdown within cardiac tissue and it is difficult to isolate undegraded native human cardiac troponin-I. This product is greater than 70% intact and hence proteolytic fragments can be observed.
  • FormLiquid
  • Additional NotesNative Human Cardiac Troponin-ISDS PAGE profile of a typical recombinant Troponin-I preparation (3.8ug) showed a major band at 24kDa and a minor band attributable to Troponin-I dimer. Please note: Troponin-I is very susceptible to proteolytic breakdown within cardiac tissue and it is difficult to isolate undegraded native human cardiac troponin-I. This product is greater than 70% intact and hence proteolytic fragments can be observed.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.

    8 M Urea, 50 mM Tris.HCl, 15 mM ß-mercaptoethanol, 1 mM EDTA (pH 8.0)

General info

  • Alternative names
    • cardiac muscle
    • Cardiac troponin I
    • Cardiomyopathy, familial hypertrophic, 7, included
    • CMD1FF
    • CMD2A
    • CMH7
    • cTnI
    • Familial hypertrophic cardiomyopathy 7
    • MGC116817
    • RCM1
    • Tn1
    • Tni
    • TNN I3
    • TNNC 1
    • TNNC1
    • TNNI3
    • TNNI3
    • TNNI3_HUMAN
    • Troponin I
    • Troponin I cardiac
    • Troponin I cardiac muscle
    • Troponin I cardiac muscle isoform
    • Troponin I type 3 cardiac
    • troponin I, cardiac 3
    • TroponinI
    • Ttroponin I type 3 (cardiac)
    see all
  • FunctionTroponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Involvement in diseaseDefects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similaritiesBelongs to the troponin I family.
  • Target information above from: UniProt accession P19429 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Cardiac Troponin I full length protein images

  • Cardiac Troponin I full length protein (ab9936) at 2.7 µg (Coomassie blue staining of SDS gel)

    Performed under reducing conditions.

    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa (possible dimer).

References for Cardiac Troponin I full length protein (ab9936)

ab9936 has not yet been referenced specifically in any publications.

Product Wall

I have been in contact with the lab who have confirmed that ab9936 is pure troponin-I and it does not contain Troponin-C. I would try and use this as the standard.
ab9919 is pure skeletal muscle troponin-I (no troponin-C) and therefore would be go...

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Troponin-I can only be purified in urea containing buffers. In the absence of urea, at concentrations above ug/ml it precipitates and/or sticks to glass or plastic war. Even in the presence of 9 M urea it completely retains its functional ability to bi...

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Thank you for contacting us.
We have following anti Cardiac Troponin IgGs.
http://www.abcam.com/cardiac-troponin-i-antibody-ab47003.html
http://www.abcam.com/cardiac-troponin-i-antibody-284-19c7-ab19615.html
http://www.abcam.com/cardia...

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Thank you very much for your call. As discussed I am happy to send you this confirmation:

Abcam takes product quality seriously and I would like to reassure you that we investigate all customer complaints.In addition to our in housequality co...

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Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which gu...

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Thank you for your reply.

Further investigation into this protein revealed that this is very tightly bound and hydrophobic (http://www.rcsb.org/pdb/explore/explore.do?structureId=1MXL). As such the full peptide will need to be stored and use...

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Thank you for contacting Abcam.

I would not recommend using a product in ELISA with a storage buffer which contains8 M Urea, 15 mM ß-mercaptoethano as I would be wary of the buffer denaturing your antibody. I would recommend using a pe...

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Thank you for contacting us.

The purity is determined by observation of a stained SDS-PAGE gel. The information I received from our source of the cardiac troponin protein is as follows:

Purity is estimated visually from the SDS PA...

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Thank you for your inquiry. I heard back from the supplying lab that this protein is purified using ion-exchange and two rounds of calcium-dependent troponin-C affinity chromatography in 8M urea solution. Therefore, little else binds to the TnC ...

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Thank you for your email. The ab9936 product is a peptide which can be used to block antibodies ab7829, ab8290 and ab8291. This peptide can be used to block these antibodies irrespective of applications e.g. It is suitable to use with antibodies...

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