Anti-Cardiac Troponin T antibody [0.N.590] (ab33589)

Overview

  • Product nameAnti-Cardiac Troponin T antibody [0.N.590]
    See all Cardiac Troponin T primary antibodies
  • Description
    Mouse monoclonal [0.N.590] to Cardiac Troponin T
  • SpecificityRecognizes the cardiac isoform of Troponin T (cTnT).
  • Tested applicationsSuitable for: WB, IHC-P, Electron Microscopy, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Dog, Human, Pig, Goldfish
    Predicted to work with: Sheep, Cat, Turkey, Zebrafish
  • Immunogen

    Synthetic peptide:

    FMPNLVPPKI

    (5' region of the cardiac gene exon 10),corresponding to Rabbit Cardiac Troponin T

  • EpitopeTnT4R Epitope.
  • Positive control
    • Cardiac muscle

Properties

Applications

Our Abpromise guarantee covers the use of ab33589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
Electron Microscopy
ICC/IF
  • Application notesElectron Microscopy: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 1 - 2 µg/ml.
    WB: 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTroponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
    • Tissue specificityHeart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
    • Involvement in diseaseDefects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
      Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
      Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
    • Sequence similaritiesBelongs to the troponin T family.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cardiac muscle troponin T antibody
      • Cardiomyopathy dilated 1D (autosomal dominant) antibody
      • Cardiomyopathy hypertrophic 2 antibody
      • CMD1D antibody
      • CMH2 antibody
      • CMPD2 antibody
      • cTnT antibody
      • LVNC6 antibody
      • MGC3889 antibody
      • OTTHUMP00000033864 antibody
      • OTTHUMP00000033865 antibody
      • OTTHUMP00000033866 antibody
      • OTTHUMP00000033867 antibody
      • OTTHUMP00000033870 antibody
      • OTTHUMP00000218095 antibody
      • RCM3 antibody
      • TNNT 2 antibody
      • TNNT2 antibody
      • TNNT2_HUMAN antibody
      • TnTc antibody
      • Troponin T cardiac muscle antibody
      • Troponin T type 2 (cardiac) antibody
      • Troponin T type 2 cardiac antibody
      • Troponin T, cardiac muscle antibody
      • Troponin T2 cardiac antibody
      see all

    References for Anti-Cardiac Troponin T antibody [0.N.590] (ab33589)

    This product has been referenced in:
    • Yin N  et al. Islet-1 promotes the cardiac-specific differentiation of mesenchymal stem cells through the regulation of histone acetylation. Int J Mol Med 33:1075-82 (2014). WB . Read more (PubMed: 24604334) »
    • Zamperone A  et al. Isolation and characterization of a spontaneously immortalized multipotent mesenchymal cell line derived from mouse subcutaneous adipose tissue. Stem Cells Dev 22:2873-84 (2013). Mouse . Read more (PubMed: 23777308) »

    See all 8 Publications for this product

    Product Wall

    Thank you for contacting us.

    The epitope sequence of antibody ab33589 has been updated.

    The epitope sequence information of ab998 and ab106076 is proprietary, we unfortunately are not able to disclose this information. These products...

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    Are you interested in an antibody against a different cardiac marker such as GATA4? The antibody ab86371 looks good.

    Click here (or use the following: http://www.abcam.com/index.html?datasheet=86371).

    The antibody ab8295, which yo...

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    Thank you for sending the sample and protocol details. I think your protocol is good and that the best option now is to replace the antibodies with different antibodies. If you have two in mind, please send the catalogue numbers. If not, I will be happ...

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    Thank you for bringing this to our attention. If I cannot offer a useful suggestion to improve your results, and if the antibodies are covered by our guarantee, I will send replacements.

    I will first need a few details of your experiment and...

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    Thank you for your call today. The cardiac troponin T antibody ab33589 should recognize the fetal chick isoform, as the immunogen is 100% homologous to a section of the fetal protein based on the SwissProt reference- http://www.uniprot.or...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (heart, ventricular tissue)
    Specification heart, ventricular tissue
    Gel Running Conditions Reduced Denaturing (4~12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Username

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    Verified customer

    Submitted Feb 02 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"