Anti-Cardiac Troponin T antibody [1C11] (ab8295)

Overview

  • Product nameAnti-Cardiac Troponin T antibody [1C11]
    See all Cardiac Troponin T primary antibodies
  • Description
    Mouse monoclonal [1C11] to Cardiac Troponin T
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, WB, ELISA, IHC-FoFr, IP, Other, IHC-P, Sandwich ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    Other Immunogen Type corresponding to Human Cardiac Troponin T aa 171-190.
    Database link: P45379

  • General notes

    This antibody detects Troponin T in human cardiac muscle. No cross-reaction with skeletal troponin T, cTnI and TnC.

Properties

Applications

Our Abpromise guarantee covers the use of ab8295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 18593559
IHC-Fr Use at an assay dependent concentration. PubMed: 17916803
AP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 35.4 kDa. PubMed: 17916803
ELISA Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 19859557
IP Use at an assay dependent concentration.
Other Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [7G7] to Cardiac Troponin T (ab10223) and Mouse monoclonal [7e7] to Cardiac Troponin T (HRP) (ab24452) and Mouse monoclonal [9G6] to Cardiac Troponin T (HRP) (ab8296). Can be used as Capture or Detection antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 18593559

Target

  • FunctionTroponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Tissue specificityHeart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
  • Involvement in diseaseDefects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
  • Sequence similaritiesBelongs to the troponin T family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cardiac muscle troponin T antibody
    • Cardiomyopathy dilated 1D (autosomal dominant) antibody
    • Cardiomyopathy hypertrophic 2 antibody
    • CMD1D antibody
    • CMH2 antibody
    • CMPD2 antibody
    • cTnT antibody
    • LVNC6 antibody
    • MGC3889 antibody
    • OTTHUMP00000033864 antibody
    • OTTHUMP00000033865 antibody
    • OTTHUMP00000033866 antibody
    • OTTHUMP00000033867 antibody
    • OTTHUMP00000033870 antibody
    • OTTHUMP00000218095 antibody
    • RCM3 antibody
    • TNNT 2 antibody
    • TNNT2 antibody
    • TNNT2_HUMAN antibody
    • TnTc antibody
    • Troponin T cardiac muscle antibody
    • Troponin T type 2 (cardiac) antibody
    • Troponin T type 2 cardiac antibody
    • Troponin T, cardiac muscle antibody
    • Troponin T2 cardiac antibody
    see all

Anti-Cardiac Troponin T antibody [1C11] images

  • ab8295 staining Cardiac Troponin T in mouse cardiac tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.5% Tx100 in PBS and blocked with 1% BSA for 90 minutes at 20°C. Samples were incubated with primary antibody (1/200 in 1% goat serum, 10% BSA + 0.1% Tx100 PBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/400) was used as the secondary antibody.

    See Abreview

  • ab8295 staining Cardiac Troponin T in human pluripotent stem cell derived cardiomyocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with saponin and blocked with 5% serum for 15 minutes at room temperature. Samples were incubated with primary antibody (1/800) for 1 hour at 21°C. An Alexa Fluor®-568 goat anti-mouse IgG monoclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ab8295 staining Cardiac Troponin T in the human iPS cell derived from cardiomyocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% triton X in PBS and blocked with 1% BSA for 18 hours at 4°C. Samples were incubated with primary antibody (1/100 in 1% BSA/PBS) for 18 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Calibration curves for sandwich cTnT fluoroimmunoassay with different animal TnTs as antigen.(dark blue) canine, (blue/grey) human, (grey) mouse, (black) rat. Monoclonal antibodies: capture, ab8295 [clone 1C11], 1 µg/well, detection ab1454 [clone 7E7], 200 ng/well. Assay time, 30 min at room temperature.
  • Ab8295 staining human normal heart. Staining is localised to the cytoplasm.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-Cardiac Troponin T antibody [1C11] (ab8295)

This product has been referenced in:
  • Poggioli T  et al. Circulating Growth Differentiation Factor 11/8 Levels Decline with Age. Circ Res N/A:N/A (2015). MS ; Mouse . Read more (PubMed: 26489925) »
  • Szebényi K  et al. Generation of a Homozygous Transgenic Rat Strain Stably Expressing a Calcium Sensor Protein for Direct Examination of Calcium Signaling. Sci Rep 5:12645 (2015). IHC ; Rat . Read more (PubMed: 26234466) »

See all 24 Publications for this product

Product Wall

Application Flow Cytometry
Fixation Paraformaldehyde
Sample Human Cell (Human embryonic stem cell derived cardiomyocytes)
Specification Human embryonic stem cell derived cardiomyocytes
Permeabilization Yes - Triton
Username

Abcam user community

Verified customer

Submitted Dec 16 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Specification Human pluripotent stem cell derived cardiomyocyte
Permeabilization Yes - saponin
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 19 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Sample Human Cell (iPS cell derived cardiomyocyte)
Specification iPS cell derived cardiomyocyte
Permeabilization Yes - 0.2% triton X
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 14 2014

Application Immunoprecipitation
Immuno-precipitation step Protein G
Sample Rat Tissue lysate - other (Left Ventrical, Heart)
Specification Left Ventrical, Heart
Total protein in input 500 µg
Username

Aaron West

Verified customer

Submitted Feb 18 2014

Thank you for your e-mail.

Unfortunately we have not determined the pI for ab8295:Anti-Cardiac Troponin T antibody. I am sorry for the inconvenience.

If you need any further assistance in the future, please do not hesitate to contac...

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Thank you for contacting us.

Yes, I can confirm the ab105441is the biotinylated form of ab8295, and this antibody shows a good affinity towards the native protein as can be seen in the ELISA and IHC data.


I hope this informat...

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Thank you very much for your patience while I was investigating.


I am happy to confirm that we do have antibody pairs validated for sandwich ELISA for Troponin T, C and I. We do not have tested troponin proteins as standard. For the Trop...

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Thank you very much for contacting us with your question.

I apologize for the confusion regarding this antibody's storage conditions. We previously recommended storage at 4C for up to one year because that is how the antibody was stored by th...

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Thank you for your email.

The two antibodies will work together. The protein we do not know whether it will work in ELISA.

This is why we could give you a testing discount on the protein. This means, you would buy the protein to the...

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Thank you very much for your interest in our products.

I am happy to confirm that the ab8295 and the ab8296 are perfectly well choosen together. They will work as a sandwich pair in ELISA.

The ab9937, which you would use as a standa...

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