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Free human cardiac troponin T
Does not cross-react with skeletal muscle troponin T, cTnI or TnC.
Our Abpromise guarantee covers the use of ab10214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. PubMed: 21278734
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|AP||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 35.4 kDa.|
|Sandwich ELISA||Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Cardiac Troponin T (ab45932). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to cardiac Troponin T (ab45932) as Detection.|
|ICC||Use at an assay dependent concentration. Used at a dilution of 1/400 for 1 hr incubation on rat cardiomyocytes (see Abreview for further details).|
|Other||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. PubMed: 23066088|
ab10214 staining human spontaneous differentiating iPSC by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with PFA, permeabilized with 0.25%% Triton X-100 in PBS and blocked with 1% BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 16 hours at 4°C. An undiluted Alexa Fluor® 594-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.
This image is courtesy of an anonymous AbreviewBlocking Step: 5% Milk for 1 hour at room temperature.
ab10214 at a 1/400 dilution staining rat cardiomyocytes by immunocytochemistry. The cells were paraformaldehyde fixed and blocked with 2% serum. The antibody was incubated with the cells for 16 hours and then bound antibody was detected using an Alexa Fluor® 594 conjugated donkey Anti-Mouse IgG(H+L).
This image is courtesy of an Abreview submitted by Yiqiang Zhang.