Anti-Cardiac Troponin T antibody [9G6] (HRP) (ab8296)

Overview

  • Product nameAnti-Cardiac Troponin T antibody [9G6] (HRP)
    See all Cardiac Troponin T primary antibodies
  • Description
    Mouse monoclonal [9G6] to Cardiac Troponin T (HRP)
  • ConjugationHRP
  • Tested applicationsSuitable for: IHC-P, WB, ELISA, Other, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) (Human).

  • Epitope1-60 aa
  • Positive control
    • IHC-P: Human heart FFPE tissue sections.
  • General notes


    This antibody detects Troponin T in human cardiac muscle. No cross-reaction with skeletal troponin T, or TnC or cTnI.

Properties

Applications

Our Abpromise guarantee covers the use of ab8296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Other Use at an assay dependent concentration.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [7G7] to Cardiac Troponin T (ab10223) and Mouse monoclonal [1C11] to Cardiac Troponin T (ab8295). Can be used as Detection antibody.

Target

  • FunctionTroponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Tissue specificityHeart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
  • Involvement in diseaseDefects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
  • Sequence similaritiesBelongs to the troponin T family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cardiac muscle troponin T antibody
    • Cardiomyopathy dilated 1D (autosomal dominant) antibody
    • Cardiomyopathy hypertrophic 2 antibody
    • CMD1D antibody
    • CMH2 antibody
    • CMPD2 antibody
    • cTnT antibody
    • LVNC6 antibody
    • MGC3889 antibody
    • OTTHUMP00000033864 antibody
    • OTTHUMP00000033865 antibody
    • OTTHUMP00000033866 antibody
    • OTTHUMP00000033867 antibody
    • OTTHUMP00000033870 antibody
    • OTTHUMP00000218095 antibody
    • RCM3 antibody
    • TNNT 2 antibody
    • TNNT2 antibody
    • TNNT2_HUMAN antibody
    • TnTc antibody
    • Troponin T cardiac muscle antibody
    • Troponin T type 2 (cardiac) antibody
    • Troponin T type 2 cardiac antibody
    • Troponin T, cardiac muscle antibody
    • Troponin T2 cardiac antibody
    see all

Anti-Cardiac Troponin T antibody [9G6] (HRP) images

  • IHC image of Cardian Troponin T staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8296, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Cardiac Troponin T antibody [9G6] (HRP) (ab8296)

ab8296 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for your patience while I was investigating.


I am happy to confirm that we do have antibody pairs validated for sandwich ELISA for Troponin T, C and I. We do not have tested troponin proteins as standard. For the Trop...

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Thank you for your email.

The two antibodies will work together. The protein we do not know whether it will work in ELISA.

This is why we could give you a testing discount on the protein. This means, you would buy the protein to the...

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Thank you very much for your interest in our products.

I am happy to confirm that the ab8295 and the ab8296 are perfectly well choosen together. They will work as a sandwich pair in ELISA.

The ab9937, which you would use as a standa...

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Thank you for contacting us.

Troponin T is a part of the troponin complex, essential for muscle contaction in skeletal and cardial muscle, but not smooth muscle.

This antibody is specific to heart muscle, and does not cross react wi...

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Thank you for your email. Another suggestion is pairing ab10220 (Mouse monoclonal [6G9] to cardiac Troponin T (HRP)) with ab8295. Please take a look at the online datasheet to see if this antibody would suit your needs. If you have any additional qu...

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