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Synthetic peptide corresponding to inter-segment of Human Cardiac Troponin T.
Our Abpromise guarantee covers the use of ab125266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 35 kDa.|
|IP||Use a concentration of 2 - 5 µg/ml.|
|ELISA||Use a concentration of 0.01 - 0.1 µg/ml.|
|IHC-P||Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 24099706|
Staining for human nuclear antigen (HNA) and cardiac lineage markers α-SMA (ab66133), cTnT (ab125266) and vWF. Random field fluorescent microscopy images (40× magnification). Arrow represents double positive cell, arrow head represents HNA+ cell.