Overview

  • Product nameAnti-Cardiac Troponin T antibody
    See all Cardiac Troponin T primary antibodies
  • Description
    Rabbit polyclonal to Cardiac Troponin T
  • Tested applicationsSuitable for: WB, IP, ELISA, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Cat, Dog, Human
  • Immunogen

    Synthetic peptide corresponding to inter-segment of Human Cardiac Troponin T.

  • Positive control
    • Human Cardiac Muscle. Mouse heart tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab125266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 35 kDa.
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.
IHC-P Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
IHC-Fr Use at an assay dependent concentration. PubMed: 24099706

Target

  • FunctionTroponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Tissue specificityHeart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
  • Involvement in diseaseDefects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
  • Sequence similaritiesBelongs to the troponin T family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cardiac muscle troponin T antibody
    • Cardiomyopathy dilated 1D (autosomal dominant) antibody
    • Cardiomyopathy hypertrophic 2 antibody
    • CMD1D antibody
    • CMH2 antibody
    • CMPD2 antibody
    • cTnT antibody
    • LVNC6 antibody
    • MGC3889 antibody
    • OTTHUMP00000033864 antibody
    • OTTHUMP00000033865 antibody
    • OTTHUMP00000033866 antibody
    • OTTHUMP00000033867 antibody
    • OTTHUMP00000033870 antibody
    • OTTHUMP00000218095 antibody
    • RCM3 antibody
    • TNNT 2 antibody
    • TNNT2 antibody
    • TNNT2_HUMAN antibody
    • TnTc antibody
    • Troponin T cardiac muscle antibody
    • Troponin T type 2 (cardiac) antibody
    • Troponin T type 2 cardiac antibody
    • Troponin T, cardiac muscle antibody
    • Troponin T2 cardiac antibody
    see all

Anti-Cardiac Troponin T antibody images

  • All lanes : Anti-Cardiac Troponin T antibody (ab125266) at 1/500 dilution

    Lane 1 : Tissue lysate from Mouse heart
    Lane 2 : Tissue lysate from Mouse heart with immunizing peptide


    Predicted band size : 35 kDa
    12% SDS-PAGE
  • Staining for human nuclear antigen (HNA) and cardiac lineage markers α-SMA (ab66133), cTnT (ab125266) and vWF. Random field fluorescent microscopy images (40× magnification). Arrow represents double positive cell, arrow head represents HNA+ cell.

  • ab125266 at 1/50 dilution, for 10 minutes at RT, staining Cardiac Troponin T in Formalin-Fixed Human cardiac muscle tissue by Immunohistochemistry.

References for Anti-Cardiac Troponin T antibody (ab125266)

This product has been referenced in:
  • Jackson R  et al. Paracrine Engineering of Human Cardiac Stem Cells With Insulin-Like Growth Factor 1 Enhances Myocardial Repair. J Am Heart Assoc 4:e002104 (2015). IHC ; Mouse . Read more (PubMed: 26363004) »
  • Mayfield AE  et al. The effect of encapsulation of cardiac stem cells within matrix-enriched hydrogel capsules on cell survival, post-ischemic cell retention and cardiac function. Biomaterials 35:133-42 (2014). IHC-Fr ; Mouse . Read more (PubMed: 24099706) »

See all 2 Publications for this product

Product Wall

The main problem is the choice of secondary antibody. The one you are using, ab96977, is anti-rabbit IgA but the primary antibodies are rabbit IgG. You need an anti-rabbit IgG secondary. I recommend ab150088:

Click here (or use the following...

Read More

Thank you for contacting Abcam regarding ab125266.


Attached please find the MSDS associated with this product.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (heart)
Specification heart
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrated Buffer, pH6
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

健 蒋

Verified customer

Submitted Apr 11 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"