Anti-Cardiac Troponin T antibody (ab89221)

Overview

  • Product nameAnti-Cardiac Troponin T antibody
    See all Cardiac Troponin T primary antibodies
  • Description
    Mouse monoclonal to Cardiac Troponin T
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-255 of human Cardiac Troponin T (AAH02653) linked to a proprietary tag.

  • Positive control
    • COLO 320 HSR cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab89221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    sELISA: Use at a concentration of 10 µg/ml. The detection limit for recombinant tagged Cardiac Troponin T is approximately 1 ng/ml as a capture antibody.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 36 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTroponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
    • Tissue specificityHeart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
    • Involvement in diseaseDefects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
      Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
      Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
    • Sequence similaritiesBelongs to the troponin T family.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cardiac muscle troponin T antibody
      • Cardiomyopathy dilated 1D (autosomal dominant) antibody
      • Cardiomyopathy hypertrophic 2 antibody
      • CMD1D antibody
      • CMH2 antibody
      • CMPD2 antibody
      • cTnT antibody
      • LVNC6 antibody
      • MGC3889 antibody
      • OTTHUMP00000033864 antibody
      • OTTHUMP00000033865 antibody
      • OTTHUMP00000033866 antibody
      • OTTHUMP00000033867 antibody
      • OTTHUMP00000033870 antibody
      • OTTHUMP00000218095 antibody
      • RCM3 antibody
      • TNNT 2 antibody
      • TNNT2 antibody
      • TNNT2_HUMAN antibody
      • TnTc antibody
      • Troponin T cardiac muscle antibody
      • Troponin T type 2 (cardiac) antibody
      • Troponin T type 2 cardiac antibody
      • Troponin T, cardiac muscle antibody
      • Troponin T2 cardiac antibody
      see all

    Anti-Cardiac Troponin T antibody images

    • Anti-Cardiac Troponin T antibody (ab89221) at 5 µg/ml + immunogen at 0.2 µg
      Developed using the ECL technique

      Predicted band size : 36 kDa
      Observed band size : ~65 kDa (why is the actual band size different from the predicted?)
    • Anti-Cardiac Troponin T antibody (ab89221) at 5 µg/ml + COLO 320 HSR cell lysate at 50 µg
      Developed using the ECL technique

      Predicted band size : 36 kDa
      Observed band size : 36 kDa
      Additional bands at : ~45 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-Cardiac Troponin T antibody (ab89221)

    ab89221 has not yet been referenced specifically in any publications.

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    The antibody is raised against a recombinant fragment corresponding to amino acids 1-255 of human Cardiac Troponin T (AAH02653) linked to a proprietary tag. The epitope that the monoclonal antibody recognizes has...

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