Anti-Cardioactive Peptide antibody (ab58736)

Overview

  • Product nameAnti-Cardioactive Peptide antibody
  • Description
    Rabbit polyclonal to Cardioactive Peptide
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Drosophila melanogaster
  • Immunogen

    Synthetic peptide corresponding to N-terminal residues of Drosophila melanogaster Cardioactive Peptide

Properties

Applications

Our Abpromise guarantee covers the use of ab58736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 18 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.
ICC/IF Use at an assay dependent concentration.

Target

  • RelevanceCardioactive peptide (CCAP) is a cardioregulatory neurohormone that increases heart beat rate during adult wing inflation. This protein has no effect on beat amplitude. The effect of cardioactive peptide is both ino- and conotrophic. Cardioactive peptide has tissue specificity within the central nervous system and most neurons exhibit coexpression with burs.
  • Cellular localizationSecreted
  • Database links
    • Alternative names
      • Cardioacceleratory peptide 2a antibody
      • CCAP antibody
      • CG4910 antibody
      • Crustacean cardioactive peptide antibody
      • DmCCAP antibody
      see all

    Anti-Cardioactive Peptide antibody images

    • Drosophila third instar larvae were dissected and fixed to get a fillet preparation. Samples were fixed in formaldehyde, permeabilized using Triton X-100, blocked with 5% serum for 1 hour at 25°C and then incubated with ab58736 at a 1/500 dilution. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.
      Cardioactive Peptide shows positive staining colocalizing with neuronal marker for HRP.

      See Abreview

    References for Anti-Cardioactive Peptide antibody (ab58736)

    ab58736 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Fruit fly (Drosophila melanogaster) Cell (neuromuscular junction of drosophila larvae)
    Specification neuromuscular junction of drosophila larvae
    Fixative Formaldehyde
    Permeabilization Yes - triton x100
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Submitted Dec 28 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"