Anti-Cardiotin antibody [R2G] (ab8962)
Key features and details
- Mouse monoclonal [R2G] to Cardiotin
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig, Zebrafish
- Isotype: IgM
Overview
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Product name
Anti-Cardiotin antibody [R2G] -
Description
Mouse monoclonal [R2G] to Cardiotin -
Host species
Mouse -
Specificity
This antibody reacts with cardiotin, a mitochondrion-associated protein which is present in cardiomyocytes and skeletal muscle. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig, Zebrafish -
Immunogen
Full length native protein (purified) corresponding to Chicken Cardiotin. Total protein extract of chicken gizzard
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General notes
Mitochondrial membrane proteins have been shown to be involved in several cellular processes such as the regulation of apoptosis and the energy metabolism. Cardiotin antibodies, which are reactive with mitochondrial proteins, have proven to be early indicators of ischemia induced changes in the myocardium.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
R2G -
Myeloma
Sp2/0-Ag14 -
Isotype
IgM -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8962 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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WB |
1/25 - 1/500.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
1/25 - 1/500. |
Target
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Relevance
Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondrial membrane. The cardiotin structure exists as subunits of 60 kDa and 100 kDa. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle a-actinin, suggesting that the tetrameric configuration of the cardiotin protein structure is a transmembrane complex with the N-terminus at the cytoplasmic side of the membrane, able to interact with actin. During cardiac contractile dysfunction, the cardiotin distribution is affected in pathlogical cardiomyocytes i.e. chronic ischemic myocardium. This monoclonal antibody can be used in immunohistochemistry for the detection of a disturbed mitochondrial activity in cardiomyocytes i.e. during chronic ischemia or chronic atrial fibrillation.
Images
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Indirect immunofluorescence analysis of swine heart cells labelling cardiotin with ab8962.
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Indirect immunofluorescence analysis of swine heart cells labelling cardiotin with ab8962.
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Human atrial cardiomyocytes stained with the monoclonal cardiotin antibody R2G.
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Immunoblotting of the microsomal fraction of swine heart reveals the 100 kDa and 60 kDa cardiotin subunits after incubation with monoclonal antibodies (a) ab8963 clone SR-2, (b) ab8964 clone SR-3 and (c) ab8962 clone R2G.
Immunoblotting of the microsomal fraction of swine heart reveals the 100 kDa and 60 kDa cardiotin subunits after incubation with monoclonal antibodies (a) ab8963 clone SR-2, (b) ab8964 clone SR-3 and (c) ab8962 clone R2G. -
Immunofluorescence staining of a 7 days old zebrafish embryo
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab8962 has been referenced in 2 publications.
- Talavera-Adame D et al. Enhancement of embryonic stem cell differentiation promoted by avian chorioallantoic membranes. Tissue Eng Part A 15:3193-200 (2009). PubMed: 19364272
- Dispersyn GD et al. Dissociation of cardiomyocyte apoptosis and dedifferentiation in infarct border zones. Eur Heart J 23:849-57 (2002). PubMed: 12042006