Overview

  • Product nameAnti-Cardiotrophin 1 antibody [3G6D9]
    See all Cardiotrophin 1 primary antibodies
  • Description
    Mouse monoclonal [3G6D9] to Cardiotrophin 1
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length protein (Human). (N-terminal HisTag, 22.5 kDa protein, 200 aa residues of the human cardiotrophin and 12 additional aa residues).

Properties

Applications

Our Abpromise guarantee covers the use of ab13975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
ELISA Use at an assay dependent concentration.

Target

  • FunctionInduces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor.
  • Tissue specificityHighly expressed in heart, skeletal muscle, prostate and ovary. Lower levels in lung, kidney, pancreas, thymus, testis and small intestine. Little or no expression in brain, placenta, liver, spleen, colon or peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cardiophin 1 antibody
    • Cardiophin1 antibody
    • Cardiotrophin I antibody
    • Cardiotrophin-1 antibody
    • Cardiotrophin1 antibody
    • CardiotrophinI antibody
    • CT 1 antibody
    • CT-1 antibody
    • CT1 antibody
    • CTF 1 antibody
    • Ctf1 antibody
    • CTF1_HUMAN antibody
    see all

Anti-Cardiotrophin 1 antibody [3G6D9] images

  • IHC image of ab13975 staining in human normal heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13975, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Cardiotrophin 1 antibody [3G6D9] (ab13975)

This product has been referenced in:
  • Nogueira-Silva C  et al. The role of glycoprotein 130 family of cytokines in fetal rat lung development. PLoS One 8:e67607 (2013). Read more (PubMed: 23826327) »
  • Natal C  et al. Cardiotrophin-1 is expressed in adipose tissue and upregulated in the metabolic syndrome. Am J Physiol Endocrinol Metab 294:E52-60 (2008). Read more (PubMed: 17940213) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab13975.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"