Cardiotrophin 1 (CTF1) Human ELISA Kit (ab99996)

Overview

  • Product nameCardiotrophin 1 (CTF1) Human ELISA Kit
    See all Cardiotrophin 1 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 20 pg/ml
  • Range
    16.46 pg/ml - 12000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98.2 76% - 112%
    Serum 132.4 120% - 142%
    Plasma 101.2 70% - 134%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Cardiotrophin 1 (CTF1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Cardiotrophin 1 in serum (Human Cardiotrophin 1 concentration is pretty low in normal serum/plasma, it may not be detected in this assay), plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Cardiotrophin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Cardiotrophin 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Cardiotrophin 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Cardiotrophin 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionInduces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor.
  • Tissue specificityHighly expressed in heart, skeletal muscle, prostate and ovary. Lower levels in lung, kidney, pancreas, thymus, testis and small intestine. Little or no expression in brain, placenta, liver, spleen, colon or peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Cardiophin 1
    • Cardiophin1
    • Cardiotrophin I
    • Cardiotrophin-1
    • CardiotrophinI
    • CT 1
    • CT-1
    • CT1
    • CTF 1
    • Ctf1
    • CTF1_HUMAN
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab99996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Cardiotrophin 1 (CTF1) Human ELISA Kit images

  • Representative Standard Curve using ab99996.

Protocols

References for Cardiotrophin 1 (CTF1) Human ELISA Kit (ab99996)

ab99996 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. Sorry to reply to in English but my Spanish colleague is currently out of the office and my Spanish is unfortunately not good enough.

The source of theCardiotrophin 1 Human ELISA Kit (ab99996)informed meit isvery a...

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Thank you for your inquiry. After reviewing your standard data, I must say the curve actually looks good (see linear and log-log plots in attachments).  As you can see, the signal strength is strong, the linearity is very nice (≥0.98), the duplicate...

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Thank you very much for your patience. I've finally heard back from the senior scientist, and he has the following response (I've placed it in quotes): "To be honest, I really don’t see an issue with the standard curve as it is giving strong signal...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"