Overview

  • Product name
  • Description
    Rabbit polyclonal to CARKL
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from the N-terminal of human CARKL.

  • Positive control
    • Human liver carcinoma tissue. Extracts from Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab69920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
ELISA 1/20000.
IHC-P 1/50 - 1/100.

Target

Images

  • All lanes : Anti-CARKL antibody (ab69920) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using ab69920 at 1/50 dilution, in the presence and absence of immunising peptide.

References

This product has been referenced in:
  • Wamelink MM  et al. First two unrelated cases of isolated sedoheptulokinase deficiency: A benign disorder? J Inherit Metab Dis 38:889-94 (2015). WB . Read more (PubMed: 25647543) »
  • Haschemi A  et al. The sedoheptulose kinase CARKL directs macrophage polarization through control of glucose metabolism. Cell Metab 15:813-26 (2012). WB ; Mouse . Read more (PubMed: 22682222) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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