Overview

  • Product nameAnti-CARS antibody
    See all CARS primary antibodies
  • Description
    Mouse polyclonal to CARS
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human protein (according to NP_001742.1, aa 1 - 748)

  • Positive control
    • Human liver tissue lysate; CARS transfected 293T lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab69454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 86 kDa.

Target

  • Involvement in diseaseNote=A chromosomal aberration involving CARS is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with ALK.
  • Sequence similaritiesBelongs to the class-I aminoacyl-tRNA synthetase family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cysteine tRNA ligase antibody
    • Cars antibody
    • CysRS antibody
    • Cysteine--tRNA ligase antibody
    • Cysteinyl tRNA synthetase, cytoplasmic antibody
    • Cysteinyl-tRNA synthetase antibody
    • cytoplasmic antibody
    • MGC:11246 antibody
    • SYCC_HUMAN antibody
    see all

Anti-CARS antibody images

  • Anti-CARS antibody (ab69454) at 1/500 dilution + human liver tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 86 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-CARS antibody (ab69454) at 1/500 dilution

    Lane 1 : CARS transfected 293T lysate
    Lane 2 : Non transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 86 kDa
    Observed band size : 86 kDa

References for Anti-CARS antibody (ab69454)

ab69454 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"