Overview

  • Product name
  • Description
    Rabbit polyclonal to CARS
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide designed within internal sequence amino acids 755-804: QEQEAAKLAK MKIPPSEMFL SETDKYSKFD ENGLPTHDME GKELSKGQAK, of Human CARS (NP_001014437)

  • Positive control
    • MCF7 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 86 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/1562500.

Target

Images

  • Anti-CARS antibody (ab83256) at 1 µg/ml (in 5% skim milk/ PBS) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 86 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Zhang L  et al. A Systematic Analysis on mRNA and MicroRNA Expression in Runting and Stunting Chickens. PLoS One 10:e0127342 (2015). WB . Read more (PubMed: 26010155) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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