Recombinant
RabMAb

Anti-CARS antibody [EPR7121] (ab126714)

Overview

  • Product name
    Anti-CARS antibody [EPR7121]
    See all CARS primary antibodies
  • Description
    Rabbit monoclonal [EPR7121] to CARS
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in human CARS (P49589)

  • Positive control
    • 293T cell lysate HeLa cell lysate U87-MG cell lysate BxPC-3 cell lysate MCF-7 cell lysate human breast carcinoma tissue A549 cell lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab126714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 85 kDa.
IHC-P 1/50 - 1/100. Antigen retrieval step is recommended.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Involvement in disease
      Note=A chromosomal aberration involving CARS is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with ALK.
    • Sequence similarities
      Belongs to the class-I aminoacyl-tRNA synthetase family.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cysteine tRNA ligase antibody
      • Cars antibody
      • CysRS antibody
      • Cysteine--tRNA ligase antibody
      • Cysteinyl tRNA synthetase, cytoplasmic antibody
      • Cysteinyl-tRNA synthetase antibody
      • cytoplasmic antibody
      • MGC:11246 antibody
      • SYCC_HUMAN antibody
      see all

    Images

    • All lanes : Anti-CARS antibody [EPR7121] (ab126714) at 1/10000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : U87-MG cell lysate
      Lane 4 : BxPC-3 cell lysate
      Lane 5 : MCF-7 cell lysate
      Lane 6 : A549 cell lysate


      Predicted band size : 85 kDa
    • ab126714, at 1/50 dilution, stains CARS in paraffin-embedded human breast carcinoma tissue by immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab126714 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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