The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50.
CARS2 (Cysteinyl tRNA synthetase 2) belongs to the class-I aminoacyl-tRNA synthetase family. Aminoacyl tRNA synthetases catalyse the attachment of an amino acid to its cognate transfer RNA molecule in a highly specific two step reaction.
ab77662, at 1/10 dilution, staining CARS2 in formalin-fixed, paraffin-embedded human hepatocarcinoma tissue by immunohistochemistry, followed by a peroxidase-conjugated secondary antibody and DAB staining.