Overview

  • Product nameAnti-CASC5 antibody
    See all CASC5 primary antibodies
  • Description
    Rabbit polyclonal to CASC5
  • Tested applicationsSuitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide from a region between residue 300 and 350 of human CASC5 using the numbering given in entry NP_733468.2

  • Positive control
    • Whole cell lysate from HeLa or 293T cells

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab70537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately >300 kDa (predicted molecular weight: 265 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • RelevancePart of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Four known isoforms exist.
  • Cellular localizationAcrosome. Nucleus.
  • Database links
  • Alternative names
    • Protein D40/AF15q14 antibody
    • AF15Q14 antibody
    • ALL1 fused gene from chromosome 15q14 antibody
    • Blinkin bub linking kinetochore protein antibody
    • Cancer susceptibility candidate 5 antibody
    • Cancer susceptibility candidate gene 5 protein antibody
    • Cancer/testis antigen 29 antibody
    • CT29 antibody
    • D40 antibody
    • hKNL 1 antibody
    • hSpc105 antibody
    • KIAA1570 antibody
    • Kinetochore null 1 homolog antibody
    • Kinetochore null protein 1 antibody
    • KNL1 antibody
    see all

Anti-CASC5 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling CASC5 with ab70537 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-CASC5 antibody (ab70537) at 1/5000 dilution

    Lane 1 : Whole cell lysate from 293T cells at 50 µg
    Lane 2 : Whole cell lysate from 293T cells at 15 µg

    Developed using the ECL technique

    Predicted band size : 265 kDa
    Observed band size : >300 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • 1mg of whole cell lysate from HeLa cells was immunoprecipitated using ab70537 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent blot, 20% of the precipitate was loaded per lane, and ab70537 was used at 1ug/ml. Detection: chemiluminescence with exposure time of 3 minutes.

References for Anti-CASC5 antibody (ab70537)

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Colon carcinoma)
Permeabilization No
Specification Colon carcinoma
Fixative Methanol
Username

Dr. Eleni Petsalaki

Verified customer

Submitted Nov 17 2015

Thank you for your recent enquiries.

I am pleased to provide you with a copy of the western blot protocol used to test this antibody. Please note as previously discussed that this would be a guideline protocol only, and may require some furt...

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Thank you for your telephone call this morning. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee forW...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"