Overview

  • Product name
    Anti-Casein antibody [F20.14]
    See all Casein primary antibodies
  • Description
    Mouse monoclonal [F20.14] to Casein
  • Specificity
    This antibody does not react with alpha lactalbumin. This antibody can be used as an in vitro marker of terminally differentiated breast lumenal epithelial cells.
  • Tested applications
    Suitable for: IHC-P, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    F20.14
  • Myeloma
    x63-Ag8.653
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 28 kDa (predicted molecular weight: 22 kDa).
IHC-Fr Use at an assay dependent concentration. Fix with acetone.

Target

  • Function
    Important role in the capacity of milk to transport calcium phosphate.
    Casoxin D acts as opioid antagonist and has vasorelaxing activity mediated by bradykinin B1 receptors.
  • Tissue specificity
    Mammary gland specific. Secreted in milk.
  • Sequence similarities
    Belongs to the alpha-casein family.
  • Post-translational
    modifications
    Not glycosylated.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha casein antibody
    • alpha-S1-casein antibody
    • CASA antibody
    • CASA1_HUMAN antibody
    • Casein alpha s1 antibody
    • casein, alpha antibody
    • casein, alpha-S1, included antibody
    • Casoxin-D antibody
    • CSN1 antibody
    • CSN1S1 antibody
    • Csna antibody
    • MGC149368 antibody
    see all

Anti-Casein antibody [F20.14] images

  • ab47972 staining Casein in Human lactating breast tissue biopsy by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde and subjected to heat-mediated antigen retrieval in citrate buffer (pH 6.0) prior to blocking with 1% normal serum containing 0.05% Tween for 30 minutes at room temperature. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour. A biotin-conjugated Donkey anti-Mouse IgG was used as the secondary antibody, diluted 1/250.

    See Abreview

References for Anti-Casein antibody [F20.14] (ab47972)

This product has been referenced in:
  • Bonuccelli G  et al. The milk protein a-casein functions as a tumor suppressor via activation of STAT1 signaling, effectively preventing breast cancer tumor growth and metastasis. Cell Cycle 11:3972-82 (2012). Read more (PubMed: 23047602) »
  • Burchell J  et al. Production and characterization of monoclonal antibodies to human casein. A monoclonal antibody that cross-reacts with casein and alpha-lactalbumin. Hybridoma 4:341-50 (1985). Read more (PubMed: 3905581) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us. Unfortunately, the antibodies mentioned are not suitable to detect these (human) proteins in ELISA. However, we have the following antibodies available: 1) Beta-lactoglobulin: Unfortunately, I was unable to find a human ort...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human lactating breast biopsy)
Specification
Human lactating breast biopsy
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Blocking step
Normal serum containing 0.05% Tween as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
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Verified customer

Submitted Sep 27 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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