Overview

  • Product name
    Anti-Casein antibody [F20.14]
    See all Casein primary antibodies
  • Description
    Mouse monoclonal [F20.14] to Casein
  • Specificity
    This antibody does not react with alpha lactalbumin. This antibody can be used as an in vitro marker of terminally differentiated breast lumenal epithelial cells.
  • Tested applications
    Suitable for: IHC-P, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    F20.14
  • Myeloma
    x63-Ag8.653
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 28 kDa (predicted molecular weight: 22 kDa).
IHC-Fr Use at an assay dependent concentration. Fix with acetone.

Target

  • Function
    Important role in the capacity of milk to transport calcium phosphate.
    Casoxin D acts as opioid antagonist and has vasorelaxing activity mediated by bradykinin B1 receptors.
  • Tissue specificity
    Mammary gland specific. Secreted in milk.
  • Sequence similarities
    Belongs to the alpha-casein family.
  • Post-translational
    modifications
    Not glycosylated.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha casein antibody
    • alpha-S1-casein antibody
    • CASA antibody
    • CASA1_HUMAN antibody
    • Casein alpha s1 antibody
    • casein, alpha antibody
    • casein, alpha-S1, included antibody
    • Casoxin-D antibody
    • CSN1 antibody
    • CSN1S1 antibody
    • Csna antibody
    • MGC149368 antibody
    see all

Images

  • ab47972 staining Casein in Human lactating breast tissue biopsy by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde and subjected to heat-mediated antigen retrieval in citrate buffer (pH 6.0) prior to blocking with 1% normal serum containing 0.05% Tween for 30 minutes at room temperature. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour. A biotin-conjugated Donkey anti-Mouse IgG was used as the secondary antibody, diluted 1/250.

    See Abreview

References

This product has been referenced in:
  • Bonuccelli G  et al. The milk protein a-casein functions as a tumor suppressor via activation of STAT1 signaling, effectively preventing breast cancer tumor growth and metastasis. Cell Cycle 11:3972-82 (2012). Read more (PubMed: 23047602) »
  • Burchell J  et al. Production and characterization of monoclonal antibodies to human casein. A monoclonal antibody that cross-reacts with casein and alpha-lactalbumin. Hybridoma 4:341-50 (1985). Read more (PubMed: 3905581) »

See all 2 Publications for this product

Customer reviews and Q&As

Thank you for contacting us. Unfortunately, the antibodies mentioned are not suitable to detect these (human) proteins in ELISA. However, we have the following antibodies available: 1) Beta-lactoglobulin: Unfortunately, I was unable to find a human ort...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human lactating breast biopsy)
Specification
Human lactating breast biopsy
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Blocking step
Normal serum containing 0.05% Tween as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Sep 27 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up