Recombinant Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1961(2)] to CSNK1A1 + CSNK1A1L
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)]
See all CSNK1A1 + CSNK1A1L primary antibodies -
Description
Rabbit monoclonal [EPR1961(2)] to CSNK1A1 + CSNK1A1L -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MCF-7, HeLa, Jurkat, Rat brain lysates, RAW 264.7, PC-12 and HT-1376 cell lysates. N-GST tagged Human CSNK1A1 and CSNK1A1L full-length recombinant protein.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1961(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 39 kDa).
For unpurified use at 1/1000 - 1/10000 |
Notes |
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WB
1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 39 kDa). For unpurified use at 1/1000 - 1/10000 |
Target
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Database links
- Entrez Gene: 122011 Human
- Entrez Gene: 1452 Human
- SwissProt: P48729 Human
- SwissProt: Q8N752 Human
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Alternative names
- CSNK1A1 antibody
- CSNK1A1L antibody
Images
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All lanes : Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296) at 1/1000 dilution
Lane 1 : N-GST tagged Human CSNK1A1L full-length (aa1 to 337) recombinant protein 10ng
Lane 2 : N-GST tagged Human CSNK1A1 full-length (aa1 to 337) recombinant protein 10ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 39 kDa -
All lanes : Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296) at 1/1000 dilution (purified)
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 39 kDaBlocking and diluting buffer: 5% NFDM/TBST
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Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296) at 1/2000 dilution + RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 39 kDaBlocking and diluting buffer: 5% NFDM/TBST
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All lanes : Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296) at 1/1000 dilution (purified)
Lane 1 : Rat brain lysates
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 39 kDaBlocking and diluting buffer: 5% NFDM/TBST
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All lanes : Anti-CSNK1A1 + CSNK1A1L antibody [EPR1961(2)] (ab108296) at 1/1000 dilution (unpurified)
Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HT-1376 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 39 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab108296 has been referenced in 15 publications.
- Yamanaka S et al. A proximity biotinylation-based approach to identify protein-E3 ligase interactions induced by PROTACs and molecular glues. Nat Commun 13:183 (2022). PubMed: 35013300
- Chen X et al. Methazolamide Attenuates the Development of Diabetic Cardiomyopathy by Promoting β-Catenin Degradation in Type 1 Diabetic Mice. Diabetes 71:795-811 (2022). PubMed: 35043173
- Shi VH et al. Phosphorylation of Syntaxin-1a by casein kinase 2a regulates pre-synaptic vesicle exocytosis from the reserve pool. J Neurochem 156:614-623 (2021). PubMed: 32852799
- Nong J et al. Phase separation of Axin organizes the β-catenin destruction complex. J Cell Biol 220:N/A (2021). PubMed: 33651074
- Shen C et al. The E3 ubiquitin ligase component, Cereblon, is an evolutionarily conserved regulator of Wnt signaling. Nat Commun 12:5263 (2021). PubMed: 34489457