Recombinant
RabMAb

Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)

Overview

  • Product name
    Anti-Casein Kinase 1 alpha antibody [EPR1961(2)]
    See all Casein Kinase 1 alpha primary antibodies
  • Description
    Rabbit monoclonal [EPR1961(2)] to Casein Kinase 1 alpha
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Casein Kinase 1 alpha aa 250-350.

  • Positive control
    • MCF-7, HeLa, Jurkat, and HT-1376 cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab108296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 39 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function
      Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
      Contains 1 protein kinase domain.
    • Cellular localization
      Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus speckle. Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • Casein kinase 1 alpha 1 antibody
      • Casein kinase I isoform alpha antibody
      • CK1 antibody
      • CK1A antibody
      • CKI alpha antibody
      • CKI-alpha antibody
      • CKIa antibody
      • Clock regulator kinase antibody
      • Csnk1a1 antibody
      • Down regulated in lung cancer antibody
      • HLCDGP1 antibody
      • KC1A_HUMAN antibody
      • PRO2975 antibody
      see all

    Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] images

    • All lanes : Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : HT-1376 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 39 kDa
      Observed band size : 34 kDa (why is the actual band size different from the predicted?)

    References for Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)

    This product has been referenced in:

    See all 3 Publications for this product

    Product Wall

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse macrophage)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    15 µg
    Specification
    Mouse macrophage
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 20 2017

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (lymphoid cell)
    Gel Running Conditions
    Reduced Denaturing (10 % gel)
    Loading amount
    10 µg
    Specification
    lymphoid cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 18 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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