Anti-Casein Kinase 1 alpha (phospho T321) antibody (ab63559)
Key features and details
- Rabbit polyclonal to Casein Kinase 1 alpha (phospho T321)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Casein Kinase 1 alpha (phospho T321) antibody
See all Casein Kinase 1 alpha primary antibodies -
Description
Rabbit polyclonal to Casein Kinase 1 alpha (phospho T321) -
Host species
Rabbit -
Specificity
Detects Casein Kinase I alpha only when phosphorylated at threonine 321. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human Casein Kinase 1 alpha aa 319-323 (phospho T321).
Sequence:A-Q-Tp-P-T
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Positive control
- HuvEc cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). |
Target
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Function
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis. -
Sequence similarities
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus speckle. Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis. - Information by UniProt
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Database links
- Entrez Gene: 1452 Human
- Entrez Gene: 93687 Mouse
- Entrez Gene: 113927 Rat
- Omim: 600505 Human
- SwissProt: P48729 Human
- SwissProt: Q8BK63 Mouse
- SwissProt: P97633 Rat
- Unigene: 529862 Human
see all -
Alternative names
- Casein kinase 1 alpha 1 antibody
- Casein kinase I isoform alpha antibody
- CK1 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab63559 has not yet been referenced specifically in any publications.