Overview

  • Product nameAnti-Casein Kinase 1 delta antibody
    See all Casein Kinase 1 delta primary antibodies
  • Description
    Goat polyclonal to Casein Kinase 1 delta
  • SpecificityThis antibody is expected to recognise both reported isoforms of Casein Kinase 1 delta
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Chimpanzee, Macaque Monkey
  • Immunogen

    Synthetic peptide:

    DREERLRHSRNPATR

    from the internal region of the protein (Human)

  • Positive control
    • HEK 293 cell lysate and lysates of Human Brain

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab48031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: Use at an assay dependent dilution.
    Antibody detection limit dilution 1/64,000.

    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 47 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCasein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm.
    • Tissue specificityExpressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. In blood, highly expressed in hemopoietic cells and mature granulocytes. Also found in monocytes and lymphocytes.
    • Involvement in diseaseDefects in CSNK1D are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated on serine and threonine residues.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Casein kinase I delta isoform antibody
      • Casein kinase I isoform delta antibody
      • CKI delta antibody
      • CKI-delta antibody
      • CKId antibody
      • CKIdelta antibody
      • CSNK 1D antibody
      • CSNK1D antibody
      • HCKID antibody
      • KC1D_HUMAN antibody
      • Protein kinase CK1 delta antibody
      see all

    Anti-Casein Kinase 1 delta antibody images

    References for Anti-Casein Kinase 1 delta antibody (ab48031)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HEK 293)
    Loading amount 20 µg
    Specification HEK 293
    Treatment DMSO, 24 hours
    Gel Running Conditions Non-reduced Denaturing (10)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 07 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"