Overview

  • Product nameAnti-Casein Kinase 1 gamma 2 antibodySee all Casein Kinase 1 gamma 2 primary antibodies ...
  • Description
    Rabbit polyclonal to Casein Kinase 1 gamma 2
  • Tested applicationsELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from N-terminus of human Casein Kinase 1 gamma 2.

  • Positive control
    • HeLa and 293 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab64829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

Target

  • FunctionSerine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity). Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis.
  • Tissue specificityTestis.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Casein kinase 1, gamma 2 antibody
    • Casein kinase I isoform gamma 2 antibody
    • Casein kinase I isoform gamma-2 antibody
    • CK1g2 antibody
    • CKI gamma 2 antibody
    • CKI-gamma 2 antibody
    • CSNK1G 2 antibody
    • Csnk1g2 antibody
    • KC1G2_HUMAN antibody
    see all

Anti-Casein Kinase 1 gamma 2 antibody images

  • Lanes 1 - 2 : Anti-Casein Kinase 1 gamma 2 antibody (ab102554)
    Lanes 3 - 4 : Anti-Casein Kinase 1 gamma 2 antibody - C-terminal (ab70862)
    Lanes 5 - 6 : Anti-Casein Kinase 1 gamma 2 antibody (ab64829) at 1/500 dilution

    Lane 1 : U2OS cells expressing FLAG-tagged CK1G2 plasmid
    Lane 2 : U2OS cells expressing FLAG tag only plasmid
    Lane 3 : U2OS cells expressing FLAG-tagged CK1G2 plasmid
    Lane 4 : U2OS cells expressing FLAG only
    Lane 5 : U2OS cells expressing FLAG-tagged CK1G2 plasmid
    Lane 6 : U2OS cells expressing FLAG only

    Secondary
    Lanes 5 - 6 : HRP-conjugated Goat anti-rabbit polyclonal at 1/1000 dilution

    developed using the ECL technique

    Predicted band size : 47 kDa

    This image is courtesy of an anonymous Abreview

    See Abreview

  • All lanes : Anti-Casein Kinase 1 gamma 2 antibody (ab64829) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : 293 cell extract
    Lane 3 : 293 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 47 kDa
    Observed band size : 47 kDa
    Additional bands at : 25 kDa,34 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Casein Kinase 1 gamma 2 antibody (ab64829)

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human osteosarcoma U2OS cells)
Loading amount 90 µg
Specification Human osteosarcoma U2OS cells
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Apr 12 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"