Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)

Overview

  • Product nameAnti-Casein Kinase 2 beta antibody [EP1995Y]
    See all Casein Kinase 2 beta primary antibodies
  • Description
    Rabbit monoclonal [EP1995Y] to Casein Kinase 2 beta
  • Tested applicationsSuitable for: WB, Flow Cyt, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Horse, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C terminus of human Casein Kinase 2 beta protein

  • Positive control
    • WB: K562 cell lysate ICC/IF: HeLa cells IHC-P: Human ovary carcinoma tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-Casein Kinase 2 beta antibody (HRP) [EP1995Y] (ab200789)

Properties

Applications

Our Abpromise guarantee covers the use of ab76025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 25 kDa.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 100 - 250 mg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for IP.
  • Target

    Anti-Casein Kinase 2 beta antibody [EP1995Y] images

    • Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025) at 1/1000 dilution + K562 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 25 kDa
      Observed band size : 25 kDa
    • Immunofluorescent staining of Casein Kinase 2 beta in HeLa cells using ab76025 at a 1/100 dilution.
    • Immunohistochemical analysis of Casein Kinase 2 beta in paraffin embedded human ovary carcinoma tissue using ab76025 at a 1/100 dilution.
    • Overlay histogram showing HeLa cells stained with ab76025 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76025, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    References for Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)

    ab76025 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (U2OS cells)
    Gel Running Conditions Reduced Denaturing (4-12% Gradient Gel)
    Loading amount 50 µg
    Specification U2OS cells
    Blocking step Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 16 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (C57BL/6 Mouse splenocytes)
    Loading amount 40 µg
    Specification C57BL/6 Mouse splenocytes
    Gel Running Conditions Reduced Denaturing (10% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Tomar Ghansah

    Verified customer

    Submitted Oct 05 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Horse Tissue sections (Mammary gland tissue)
    Specification Mammary gland tissue
    Fixative Formaldehyde
    Antigen retrieval step None
    Permeabilization No
    Username

    Dr. Jan Spaas

    Verified customer

    Submitted Jun 09 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"