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Product name Anti-Casein Kinase 2 beta antibody [EP1995Y] See all Casein Kinase 2 beta primary antibodies
Rabbit monoclonal [EP1995Y] to Casein Kinase 2 beta
Tested applications Suitable for:
WB, Flow Cyt, IHC-P, ICC/IF more details Unsuitable for:
Mouse, Rat, Horse, Human
Synthetic peptide corresponding to residues near the C terminus of human Casein Kinase 2 beta protein
WB: K562 cell lysate
ICC/IF: HeLa cells
IHC-P: Human ovary carcinoma tissue
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb
® technology. RabMAb ® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternative versions available:
Anti-Casein Kinase 2 beta antibody (HRP) [EP1995Y] ( ab200789)
Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity Tissue culture supernatant
Clone number EP1995Y
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Predicted molecular weight: 25 kDa.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Use a concentration of 100 - 250 mg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/100 - 1/250.
Application notes Is unsuitable for IP.
Function Participates in Wnt signaling (By similarity). Plays a complex role in regulating the basal catalytic activity of the alpha subunit.
Sequence similarities Belongs to the casein kinase 2 subunit beta family.
Post-translational modifications Phosphorylated by alpha subunit.
Information by UniProt
Casein kinase 2 beta polypeptide antibody
Casein kinase II beta subunit antibody
Casein kinase II subunit beta antibody
Anti-Casein Kinase 2 beta antibody [EP1995Y] images
Western blot - Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025) at 1/1000 dilution + K562 cell lysate at 10 µg Secondary HRP labelled goat anti-rabbit at 1/2000 dilution Predicted band size : 25 kDa Observed band size : 25 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
Immunofluorescent staining of Casein Kinase 2 beta in HeLa cells using ab76025 at a 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
Immunohistochemical analysis of Casein Kinase 2 beta in paraffin embedded human ovary carcinoma tissue using ab76025 at a 1/100 dilution.
Flow Cytometry - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
Overlay histogram showing HeLa cells stained with ab76025 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76025, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) ( ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10 6 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025) This image is courtesy of an anonymous Abreview
Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025) at 1/1000 dilution + MEF whole cell lysate at 20 µg Secondary Alexa Fluor® 680-conjugated polyclonal goat anti-rabbit IgG at 1/5000 dilution Performed under reducing conditions. Predicted band size : 25 kDa Observed band size : 25 kDa Exposure time : 10 minutes This image is courtesy of an anonymous Abreview
Blocked for 30 minutes at 25°C.
Incubated with the primary antibody for 2 hours at 25°C in blocking buffer.
References for Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"