Anti-Casein Kinase 2 beta (phospho S209) antibody (ab12861)

Overview

  • Product name
    Anti-Casein Kinase 2 beta (phospho S209) antibody
    See all Casein Kinase 2 beta primary antibodies
  • Description
    Rabbit polyclonal to Casein Kinase 2 beta (phospho S209)
  • Specificity
    Does not cross react with Casein Kinase 2 alpha [pS360] (mouse), [pS362], [pS370], or Casein Kinase 2 beta pS2].
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic phosphopeptide (Human) from the region of casein kinase 2 that contains serine 209.

  • Positive control
    • HeLa cell lysates or K562 cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 1mg/ml BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Casein Kinase 2 beta. The final product is generated by affinity chromatography using a Casein Kinase 2 beta derived peptide that is phosphorylated at serine 209.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab12861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/1000 in 3% milk in 0.1% Tween20 (TBST). Incubate overnight. Detects a band of approximately 25 kDa. Block 1hr with 3% milk in 0.1% Tween20 (TBST).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Participates in Wnt signaling (By similarity). Plays a complex role in regulating the basal catalytic activity of the alpha subunit.
    • Sequence similarities
      Belongs to the casein kinase 2 subunit beta family.
    • Post-translational
      modifications
      Phosphorylated by alpha subunit.
    • Information by UniProt
    • Database links
    • Alternative names
      • Casein kinase 2 beta polypeptide antibody
      • Casein kinase II beta subunit antibody
      • Casein kinase II subunit beta antibody
      • CK II beta antibody
      • CK2B antibody
      • CK2N antibody
      • CSK2B antibody
      • CSK2B_HUMAN antibody
      • CSNK 2B antibody
      • csnk2b antibody
      • G5A antibody
      • MGC138222 antibody
      • MGC138224 antibody
      • Phosvitin antibody
      • Protein G5a antibody
      see all

    Images

    • Peptide Competition and Phosphatase Treatment

      Lysates prepared from HeLa cells were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda (λ) phosphatase (5), blocked with a 3% Milk-TBST buffer for one hour at room temperature, and incubated with CK2β [pS209] (ab12861) antibody for two hours at room temperature in a 3% Milk- TBST buffer, following prior incubation with: no peptide (1, 5), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphoserine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method.

      The data show that only the peptide corresponding to CK2β [pS209] blocks the signal, verifying the specificity of the antibody.

    References

    ab12861 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for your enquiry. The S209 refers to serine 209. The immunogen used to generate this antibody was a synthetic phosphopeptide (Human) from the region of casein kinase 2 that contains serine 209. The antibody has been negatively preadsorbed...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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