Anti-Casein Kinase 2 beta (phospho S209) antibody (ab12861)

Overview

  • Product nameAnti-Casein Kinase 2 beta (phospho S209) antibody
    See all Casein Kinase 2 beta primary antibodies
  • Description
    Rabbit polyclonal to Casein Kinase 2 beta (phospho S209)
  • SpecificityDoes not cross react with Casein Kinase 2 alpha [pS360] (mouse), [pS362], [pS370], or Casein Kinase 2 beta pS2].
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic phosphopeptide (Human) from the region of casein kinase 2 that contains serine 209.

  • Positive control
    • HeLa cell lysates or K562 cell lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 1mg/ml BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Casein Kinase 2 beta. The final product is generated by affinity chromatography using a Casein Kinase 2 beta derived peptide that is phosphorylated at serine 209.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab12861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000 in 3% milk in 0.1% Tween20 (TBST). Incubate overnight. Detects a band of approximately 25 kDa. Block 1hr with 3% milk in 0.1% Tween20 (TBST).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionParticipates in Wnt signaling (By similarity). Plays a complex role in regulating the basal catalytic activity of the alpha subunit.
    • Sequence similaritiesBelongs to the casein kinase 2 subunit beta family.
    • Post-translational
      modifications
      Phosphorylated by alpha subunit.
    • Information by UniProt
    • Database links
    • Alternative names
      • Casein kinase 2 beta polypeptide antibody
      • Casein kinase II beta subunit antibody
      • Casein kinase II subunit beta antibody
      • CK II beta antibody
      • CK2B antibody
      • CK2N antibody
      • CSK2B antibody
      • CSK2B_HUMAN antibody
      • CSNK 2B antibody
      • csnk2b antibody
      • G5A antibody
      • MGC138222 antibody
      • MGC138224 antibody
      • Phosvitin antibody
      • Protein G5a antibody
      see all

    Anti-Casein Kinase 2 beta (phospho S209) antibody images

    • Peptide Competition and Phosphatase Treatment

      Lysates prepared from HeLa cells were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda (λ) phosphatase (5), blocked with a 3% Milk-TBST buffer for one hour at room temperature, and incubated with CK2β [pS209] (ab12861) antibody for two hours at room temperature in a 3% Milk- TBST buffer, following prior incubation with: no peptide (1, 5), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphoserine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method.

      The data show that only the peptide corresponding to CK2β [pS209] blocks the signal, verifying the specificity of the antibody.

    References for Anti-Casein Kinase 2 beta (phospho S209) antibody (ab12861)

    ab12861 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry. The S209 refers to serine 209. The immunogen used to generate this antibody was a synthetic phosphopeptide (Human) from the region of casein kinase 2 that contains serine 209. The antibody has been negatively preadsorbed...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"