Anti-Casein Kinase 2 beta (phospho S209) antibody (ab59433)

Overview

  • Product name
    Anti-Casein Kinase 2 beta (phospho S209) antibody
    See all Casein Kinase 2 beta primary antibodies
  • Description
    Rabbit polyclonal to Casein Kinase 2 beta (phospho S209)
  • Specificity
    Detects endogenous levels of Casein Kinase 2 beta only when phosphorylated at serine 209.
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide (Human) from around the phosphorylation site of serine 209 (FKSPPV)

  • Positive control
    • Human brain tissue and HuvEc cell extracts.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/40000.

Target

Anti-Casein Kinase 2 beta (phospho S209) antibody images

  • Immunohistochemical analysis of paraffin embedded human brain tissue using ab59433 at 1/50 dilution; in the presence or absence of the phosphopeptide.
  • All lanes : Anti-Casein Kinase 2 beta (phospho S209) antibody (ab59433) at 1/500 dilution

    Lane 1 : HuvEc cell extract
    Lane 2 : HuvEc cell extract with phosphopeptide


    Predicted band size : 25 kDa
    Observed band size : 25 kDa

References for Anti-Casein Kinase 2 beta (phospho S209) antibody (ab59433)

ab59433 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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