Anti-Casein Kinase 2 beta (phospho S209) antibody (ab59433)

Overview

  • Product nameAnti-Casein Kinase 2 beta (phospho S209) antibody
    See all Casein Kinase 2 beta primary antibodies
  • Description
    Rabbit polyclonal to Casein Kinase 2 beta (phospho S209)
  • SpecificityDetects endogenous levels of Casein Kinase 2 beta only when phosphorylated at serine 209.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide (Human) from around the phosphorylation site of serine 209 (FKSPPV)

  • Positive control
    • Human brain tissue and HuvEc cell extracts.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAffinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/40000.

Target

Anti-Casein Kinase 2 beta (phospho S209) antibody images

  • Immunohistochemical analysis of paraffin embedded human brain tissue using ab59433 at 1/50 dilution; in the presence or absence of the phosphopeptide.
  • All lanes : Anti-Casein Kinase 2 beta (phospho S209) antibody (ab59433) at 1/500 dilution

    Lane 1 : HuvEc cell extract
    Lane 2 : HuvEc cell extract with phosphopeptide


    Predicted band size : 25 kDa
    Observed band size : 25 kDa

References for Anti-Casein Kinase 2 beta (phospho S209) antibody (ab59433)

ab59433 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"