Anti-Caseine Kinase 1 alpha antibody (ab64939)

Overview

  • Product nameAnti-Caseine Kinase 1 alpha antibody
    See all Casein Kinase 1 alpha primary antibodies
  • Description
    Rabbit polyclonal to Caseine Kinase 1 alpha
  • Specificityab64939 detects endogenous levels of total Caseine Kinase 1 alpha protein.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human) from an internal sequence.

  • Positive control
    • Human lung carcinoma tissue, HeLa cells and HeLa cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab64939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
ICC/IF
  • Application notesELISA: 1/20000.
    ICC/IF: 1/500 - 1/1000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCasein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
      Contains 1 protein kinase domain.
    • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus speckle. Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • Casein kinase 1 alpha 1 antibody
      • Casein kinase I isoform alpha antibody
      • CK1 antibody
      • CK1A antibody
      • CKI alpha antibody
      • CKI-alpha antibody
      • CKIa antibody
      • Clock regulator kinase antibody
      • Csnk1a1 antibody
      • Down regulated in lung cancer antibody
      • HLCDGP1 antibody
      • KC1A_HUMAN antibody
      • PRO2975 antibody
      see all

    Anti-Caseine Kinase 1 alpha antibody images

    • This image shows human lung carcinoma tissue stained with ab64939 at 1/50 dilution; with and without the immunizing peptide.
    • This image shows HeLa cells stained with ab64939 at 1/500 dilution; with and without the immunising peptide.
    • All lanes : Anti-Caseine Kinase 1 alpha antibody (ab64939) at 1/500 dilution

      Lane 1 : extracts from HeLa cells (5-30µg total protein)
      Lane 2 : extracts from HeLa cells (5-30µg total protein) with 5-10µg of the immunising peptide.


      Predicted band size : 39 kDa
      Observed band size : 39 kDa

    References for Anti-Caseine Kinase 1 alpha antibody (ab64939)

    ab64939 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"