CASK antibody (ab11343)

Product Name 

CASK antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to CASK

Immunogen 

Synthetic peptide: KSSPQIRNPPSDAVQRA conjugated to KLH, corresponding to amino acids 393-409 of Human CASK.
BLAST 'KSSPQIRNPPSDAVQRA' with or

Reacts with 
(species key)

Ms, Rat, Dog

Specificity 

Anti-CASK/Lin2 specifically recognizes human CASK/Lin2 by immunoblotting and immunoprecipitation (112 kDa).
Staining of Cask/Lin2 by immunoblotting is inhibited by the immunizing peptide.
Additional bands may be detected in some extract preparations.

Tested applications 
(see key)

IP, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
IP: Use at a concentration of 5 - 10 µg/ml.
WB: 1/200. Detects a band of approximately 112 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic (Potential).

Research areas 

Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases

Relevance 

Calmodulin sensitive kinase (CASK/mLIN-2) is a ~112-kDa member of the membrane associated quanylate kinase (MAGUK) protein family with multiple protein binding domains. These include an N-terminal calmodulin kinase (CaMKII) domain and C-terminal PDZ, SH3-, and quanylate kinase domains. CASK possesses both tyrosine kinase and serine/threonine kinase activity. In addition, CASK can form a heterotrimeric complex with Mint (X11or mLIN-10) and Veli (mLIN-7) proteins which is predicted to participate in plasma membrane receptor localization. CASK is localized to neuronal synapses and also interacts with syndecan and neurexin. The guanylate kinase domain of CASK has been shown to interact with the T-box transcription factor, Tbr-1, as a coactivator to induce transcription of genes that contain T-elements.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.400 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilutions should be discarded if not used within 12 hours.

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