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Products:Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Overview
Product name
Anti-CASK antibody
See all CASK products (5) ...
Description
Rabbit polyclonal to CASK
Specificity
Anti-CASK/Lin2 specifically recognizes human CASK/Lin2 by immunoblotting and immunoprecipitation (112 kDa). Staining of Cask/Lin2 by immunoblotting is inhibited by the immunizing peptide. Additional bands may be detected in some extract preparations.
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Dog
Immunogen
Synthetic peptide: KSSPQIRNPPSDAVQRA conjugated to KLH, corresponding to amino acids 393-409 of Human CASK.
KSSPQIRNPP SDAVQRA
General notes
Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilutions should be discarded if not used within 12 hours.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/200Detects a band of approximately 112 kDa.
IP: Use a concentration of 5 - 10 µg/ml.
Target
Function
Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1.
Tissue specificity
Ubiquitous. Expression is significantly greater in brain relative to kidney, lung, and liver and in fetal brain and kidney relative to lung and liver.
Involvement in disease
Defects in CASK are the cause of mental retardation X-linked CASK-related (MRXCASK) [MIM:300749]. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Patients with mental retardation X-linked CASK-related can manifest a severe phenotype consisting of severe intellectual deficit, congenital or postnatal microcephaly, disproportionate brainstem and cerebellar hypoplasia (MICPCH Syndrome). A milder phenotype consists of mental retardation alone or associated with nystagmus.
Defects in CASK are the cause of FG syndrome type 4 (FGS4) [MIM:300422]. FG syndrome (FGS) is an X-linked disorder characterized by mental retardation, relative macrocephaly, hypotonia and constipation.
Sequence similarities
In the N-terminal section; belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 2 L27 domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain.
Contains 1 SH3 domain.
Domain
The first L27 domain binds DLG1 and the second L27 domain probably binds LIN7.
The protein kinase domain mediates the interaction with FCHSD2.
Cellular localization
Nucleus. Cytoplasm. Cell membrane.
Target information above from: UniProt accessionO14936
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CAGH39 antibody
- Calcium/calmodulin dependent serine protein kinase antibody
- Calcium/calmodulin dependent serine protein kinase (MAGUK family) antibody
see all
Database links
- Entrez Gene: 12361 Mouse
- Entrez Gene: 29647 Rat
- SwissProt: O70589 Mouse
- SwissProt: Q62915 Rat
- Unigene: 327591 Mouse
- Unigene: 474948 Mouse
- Unigene: 72627 Rat
References for Anti-CASK antibody (ab11343)
ab11343 has not yet been referenced specifically in any publications.
Publishing research using ab11343? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"