Overview

  • Product nameAnti-CASK antibody [S56A-50]
    See all CASK primary antibodies
  • Description
    Mouse monoclonal [S56A-50] to CASK
  • Tested applicationsSuitable for: WB, IP, IHC-P, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Xenopus laevis, Zebrafish
  • Immunogen

    Fusion protein: NSFYGDPPEE LPDFSEDPTS SGLLAAERAV SQVLDSLEEI HALTDCSEKD LDFLHSVFQD QHLHTLLDLY DKINTKSSPQ IRNPPSDAV, corresponding to amino acids 318-415 of Rat CASK (accession number Q62915).

  • Positive control
    • Rat brain tissue extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab99039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 103 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionMultidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1.
  • Tissue specificityUbiquitous. Expression is significantly greater in brain relative to kidney, lung, and liver and in fetal brain and kidney relative to lung and liver.
  • Involvement in diseaseDefects in CASK are the cause of mental retardation X-linked CASK-related (MRXCASK) [MIM:300749]. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Patients with mental retardation X-linked CASK-related can manifest a severe phenotype consisting of severe intellectual deficit, congenital or postnatal microcephaly, disproportionate brainstem and cerebellar hypoplasia (MICPCH Syndrome). A milder phenotype consists of mental retardation alone or associated with nystagmus.
    Defects in CASK are the cause of FG syndrome type 4 (FGS4) [MIM:300422]. FG syndrome (FGS) is an X-linked disorder characterized by mental retardation, relative macrocephaly, hypotonia and constipation.
  • Sequence similaritiesIn the N-terminal section; belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 2 L27 domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
    Contains 1 SH3 domain.
  • DomainThe first L27 domain binds DLG1 and the second L27 domain probably binds LIN7.
    The protein kinase domain mediates the interaction with FCHSD2.
  • Cellular localizationNucleus. Cytoplasm. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAGH39 antibody
    • Caki antibody
    • Calcium/calmodulin dependent serine protein kinase antibody
    • Calcium/calmodulin dependent serine protein kinase (MAGUK family) antibody
    • Calcium/calmodulin dependent serine protein kinase membrane associated guanylate kinase antibody
    • Calcium/calmodulin-dependent serine protein kinase antibody
    • CAMGUK antibody
    • CAMGUK protein antibody
    • CAMGUK, drosophila, homolog of antibody
    • casK antibody
    • CMG antibody
    • CSKP_HUMAN antibody
    • DXPri1 antibody
    • DXRib1 antibody
    • FGS4 antibody
    • FLJ22219 antibody
    • FLJ31914 antibody
    • hCASK antibody
    • LIN 2 antibody
    • Lin 2 homolog antibody
    • LIN2 antibody
    • Lin2 homolog antibody
    • MICPCH antibody
    • MRXSNA antibody
    • Pals3 antibody
    • Peripheral plasma membrane protein CASK antibody
    • Protein lin-2 homolog antibody
    • TNRC8 antibody
    • Trinucleotide repeat containing 8 antibody
    • Vertebtate LIN2 homolog antibody
    see all

Anti-CASK antibody [S56A-50] images



  • Predicted band size : 103 kDa
    Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: CASK knockout HAP1 cell lysate (20 µg)
    Lane 3: Rat brain tissue lysate (20 µg)
    Lane 4: SH-SY5Y cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab99039 observed at 110 kDa. Red - loading control, ab18251, observed at 50 kDa.

    ab99039 was shown to specifically react with CASK when CASK knockout samples were used. Wild-type and CASK knockout samples were subjected to SDS-PAGE. ab99039 and ab18251 (loading control to alpha Tubulin) diluted to 1/500 and 1/10000 respective were incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • ab99039 staining CASK in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).

  • Anti-CASK antibody [S56A-50] (ab99039) at 1/1000 dilution + Rat brain membrane tissue lysate

    Predicted band size : 103 kDa
  • Overlay histogram showing SH-SY5Y cells stained with ab99039 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab99039, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References for Anti-CASK antibody [S56A-50] (ab99039)

This product has been referenced in:
  • Jiang M  et al. Changes in tension regulates proliferation and migration of fibroblasts by remodeling expression of ECM proteins. Exp Ther Med 12:1542-1550 (2016). WB ; Human . Read more (PubMed: 27588075) »

See 1 Publication for this product

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