Anti-Caspase-1 antibody [14F468] (ab14367)

Overview

  • Product nameAnti-Caspase-1 antibody [14F468]
    See all Caspase-1 primary antibodies
  • Description
    Mouse monoclonal [14F468] to Caspase-1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Pig
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-1 aa 371-390.
    Sequence:

    RKVRFSFEQPDGRAQMPTTE


    Database link: P29466

  • Positive control
    • HeLa cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab14367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 45 kDa.
IHC-P 1/10 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionThiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
  • Tissue specificityExpressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Post-translational
    modifications
    The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CASP-1 antibody
    • CASP1 antibody
    • CASP1_HUMAN antibody
    • Caspase 1 antibody
    • Caspase-1 subunit p10 antibody
    • ICE antibody
    • IL-1 beta-converting enzyme antibody
    • IL-1BC antibody
    • IL1 beta converting enzyme antibody
    • IL1B convertase antibody
    • Interleukin 1 beta convertase antibody
    • Interleukin 1B converting enzyme antibody
    • Interleukin-1 beta convertase antibody
    • Interleukin-1 beta-converting enzyme antibody
    • p45 antibody
    see all

Anti-Caspase-1 antibody [14F468] images

  • Immunohistochemical analysis of formalin-fixed, paraffin embedded normal Human lung tissue labeling Caspase 1 with ab14367 at 5 ug/ml. In this representative lung section, different type of cells including pseudostratified columnar epithelium of bronchiole and the simple squamous epithelium of alveoli may be seen to develop immunoreactivity for Caspase 1. [10X Magnification]

  • Immunohistochemical analysis of formalin-fixed, paraffin embedded normal Human skin tissue labeling Caspase 1 with ab14367 at 5 ug/ml. Strong cytoplasmic/nuclear staining developed in all the epidermal cells, blood vessels and some cells of the dermal connective tissues layer. [10X Magnification]

  • Immunohistochemical analysis of formalin-fixed, paraffin embedded normal Human colon tissue labeling Caspase 1 with ab14367 at 5 ug/ml. Distinct cytoplasmic staining along with some nuclear positivity was observed in crypts/mucosa, and staining was found to be more intense in the absorptive columnar epithelial cells. [10X Magnification]

  • Immunohistochemical analysis of formalin-fixed, paraffin embedded Human adenocarcinoma of the rectum tissue labeling Caspase 1 with ab14367 at 5 ug/ml. peroxidase-conjugate and DAB chromogen.

     

    Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20

    min followed by cooling at RT for 20 min.

  • Immunohistochemical analysis of paraffin embedded Human ovarian cancer tissue labeling Caspase 1 with ab14367 at 5 ug/ml.

     

     

  • Western blot analysis of Caspase-1 in HeLa cell lysate using ab14367 at 0.5 ug/ml.

    Western blot analysis of Caspase-1 in HeLa cell lysate using ab14367 at 0.5 ug/ml.

References for Anti-Caspase-1 antibody [14F468] (ab14367)

ab14367 has not yet been referenced specifically in any publications.

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