• Product name
  • Description
    Rabbit polyclonal to Caspase-1
  • Specificity
    This antibody reacts with the 45 kDa protein Caspase-1.
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Caspase-1 (internal sequence). Immunogen is within the p20 subunit.
    Database link: P29466

  • Positive control
    • Placenta



Our Abpromise guarantee covers the use of ab1872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. PubMed: 21228274
ICC/IF 1/200.


  • Function
    Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
  • Tissue specificity
    Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
  • Sequence similarities
    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Post-translational
    The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • CASP-1 antibody
    • CASP1 antibody
    • CASP1_HUMAN antibody
    • Caspase 1 antibody
    • Caspase-1 subunit p10 antibody
    • ICE antibody
    • IL-1 beta-converting enzyme antibody
    • IL-1BC antibody
    • IL1 beta converting enzyme antibody
    • IL1B convertase antibody
    • Interleukin 1 beta convertase antibody
    • Interleukin 1B converting enzyme antibody
    • Interleukin-1 beta convertase antibody
    • Interleukin-1 beta-converting enzyme antibody
    • p45 antibody
    see all


  • ab1872 staining Caspase-1 in mouse immortalised bone marrow-derived macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 (0.1%) and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1:200 in PBS + 0.1% BSA/5% goat serum) for 1 hour at 20°C. An anti-rabbit Alexa Fluor®568 (1/500) ab175471) was used as the secondary antibody.

    See Abreview

  • ab1872 staining Caspase-1 in formalin-fixed, paraffin-embedded Human placenta tissue by Immunohistochemistry.

  • Immunofluorescence analysis of caspase-1 in H9c2 cardiomyocytes treated with control, medium and high glucose using ab1872 (1:50) ; scale bar: 20 µm. 
    Ctrl: control glucose, 5.6 mM glucose for 36 h;
    MG: medium glucose, 25 mM glucose for 36 h;
    HG: high glucose, 33.3 mM glucose for 36 h; 



This product has been referenced in:
  • Xiao N  et al. Ginsenoside Rg5 Inhibits Succinate-Associated Lipolysis in Adipose Tissue and Prevents Muscle Insulin Resistance. Front Pharmacol 8:43 (2017). Read more (PubMed: 28261091) »
  • Cao S  et al. Melatonin-mediated mitophagy protects against early brain injury after subarachnoid hemorrhage through inhibition of NLRP3 inflammasome activation. Sci Rep 7:2417 (2017). WB . Read more (PubMed: 28546552) »

See all 10 Publications for this product

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Rhesus monkey Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA
Blocking step
Serum as blocking agent for 8 minute(s) · Concentration: 25% · Temperature: 37°C

Ms. Maria Daskalaki

Verified customer

Submitted Nov 01 2015

Western blot
Rhesus monkey Tissue lysate - whole (lympoid node)
Gel Running Conditions
Reduced Denaturing (15% gel)
Loading amount
40 µg
lympoid node
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 03 2015

Thank you for this clarification. Our datasheet and tissue specificity is based off of the findings reported in the literature. However, should we find that brain expression becomes reported in the literature we would make an effort to update our records.

Thank you for your reply. I apologize for my error, this is a caspase 1 antibody. The prostate cancer sounds like a good control. We also have lsited placenta. One recommendation might be to switch your blocking agent and increase the time to 2 hours. ...

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Thank you for contacting us.
For IHC on Frozen sections we recommend to use this product without any antigen reetrieval and develop using the ABC method. The primary antibody should be used at a dilution of 1/25 to 1/50 overnight at 4 degrees. For...

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Thank you for contacting us.
I am sorry to hear you are experiencing difficulties with our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
I am attaching our que...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Mouse Cell (immortalised bone marrow-derived macrophages)
immortalised bone marrow-derived macrophages
Yes - 0.1% Triton X100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. James Harris

Verified customer

Submitted Feb 01 2011

There is no antibody specific only for the activated form. As the antibody will recognize the same sequence despite whether it is in the pro-form or processed form. Pro-caspase antibodies are specific as the active version will not have the specific p...

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Thank you for your reply. The protocol information you have sent me looks very good and I do not think you need to change any of those steps. I enclose a link to a protocol for nuclear extraction: http://www.abcam.com/assets/pdf/protocols/Nuclear%2...

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I was informed this morning that we do not know what dilution was used when ab1872 was tested in WB as this was reported to work by a customer who didn't specify the dilution which was optimal. I would therefore recommend to try a range of dilutions (...

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