The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use at an assay dependent concentration. PubMed: 21228274
Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
Belongs to the peptidase C14A family. Contains 1 CARD domain.
The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
Immunocytochemistry/ Immunofluorescence - Anti-Caspase-1 antibody (ab1872)This image is courtesy of an Abreview submitted by James Harris
ab1872 staining Caspase-1 in mouse immortalised bone marrow-derived macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 (0.1%) and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1:200 in PBS + 0.1% BSA/5% goat serum) for 1 hour at 20°C. An anti-rabbit Alexa Fluor®568 (1/500) ab175471) was used as the secondary antibody.
Immunofluorescence analysis of caspase-1 in H9c2 cardiomyocytes treated with control, medium and high glucose using ab1872 (1:50) ; scale bar: 20 µm. Ctrl: control glucose, 5.6 mM glucose for 36 h; MG: medium glucose, 25 mM glucose for 36 h; HG: high glucose, 33.3 mM glucose for 36 h;
Ding HG et al. Hypercapnia induces IL-1ß overproduction via activation of NLRP3 inflammasome: implication in cognitive impairment in hypoxemic adult rats. J Neuroinflammation15:4 (2018).
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