Anti-Caspase-1 antibody (ab53175)

Overview

  • Product name
  • Description
    Rabbit polyclonal to Caspase-1
  • Specificity
    ab53175 detects endogenous levels of fragment of activated Caspase-1 resulting from cleavage adjacent to Asp116. Ab53175 also detects the uncleaved form in WB.
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-1 (cis ). Cleaved-Asp210.
    Database link: P29466

  • Positive control
    • IHC-P: Human lung carcinoma tissue. WB: K562 and HepG2 cell lysates treated with adriamycin.

Properties

Applications

Our Abpromise guarantee covers the use of ab53175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000.

25 kDa (cleaved), 45 kDa (pro-peptide)

IHC-P 1/50 - 1/100.
ELISA 1/10000.

Target

  • Function
    Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
  • Tissue specificity
    Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
  • Sequence similarities
    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Post-translational
    modifications
    The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CASP-1 antibody
    • CASP1 antibody
    • CASP1_HUMAN antibody
    • Caspase 1 antibody
    • Caspase-1 subunit p10 antibody
    • ICE antibody
    • IL-1 beta-converting enzyme antibody
    • IL-1BC antibody
    • IL1 beta converting enzyme antibody
    • IL1B convertase antibody
    • Interleukin 1 beta convertase antibody
    • Interleukin 1B converting enzyme antibody
    • Interleukin-1 beta convertase antibody
    • Interleukin-1 beta-converting enzyme antibody
    • p45 antibody
    see all

Images

  • ab53175 at 1/50 dilution staining Caspase-1 in human lung carcinoma by Immunohistochemsitry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

  • All lanes : Anti-Caspase-1 antibody (ab53175)

    Lane 1 : K562 cell lysate treated with adriamycin
    Lane 2 : HepG2 cell lysate treated with adriamycin

References

ab53175 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

That is good news, thanks for the update. If you have any trouble with the IF stain, please contact us. Good luck!

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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